Mutant fragments of ospa and methods and uses relating thereto

ABSTRACT

The present invention relates to compositions and methods for the prevention and treatment of Borrelia infection. Particularly, the present invention relates to a polypeptide comprising a hybrid C-terminal fragment of an outer surface protein A (OspA), a nucleic acid coding the same, an antibody specifically binding the same, a pharmaceutical composition (particularly for use as a medicament or in a method of treating or preventing a Borrelia infection) comprising the polypeptide and/or the nucleic acid and/or the antibody, a method of treating or preventing a Borrelia infection and a method of immunizing a subject.

RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No. 16/990,023, which is a divisional of U.S. application Ser. No. 15/944,835, now U.S. Pat. No. 10,766,931, filed Apr. 4, 2018, which is a continuation of U.S. application Ser. No. 15/110,151, now U.S. Pat. No. 9,975,927, filed Jul. 7, 2016, which is a national stage filing under 35 U.S.C. § 371 of international application PCT/EP2015/050365, filed Jan. 9, 2015, which was published under PCT Article 21(2) in English, the content of each of which are incorporated by reference herein in its entirety.

REFERENCE TO AN ELECTRONIC SEQUENCE LISTING

The contents of the electronic sequence listing (10422701200503-SEQ-NTJ.xml; Size: 84,534 bytes; and Date of Creation: Jan. 3, 2023) is herein incorporated by reference in its entirety.

FIELD OF THE INVENTION

The present invention relates to compositions and methods for the prevention and treatment of Borrelia infection. Particularly, the present invention relates to a polypeptide comprising a hybrid C-terminal fragment of an outer surface protein A (OspA), a nucleic acid coding the same, an antibody specifically binding the same, a pharmaceutical composition (particularly for use as a medicament or in a method of treating or preventing a Borrelia infection) comprising the polypeptide and/or the nucleic acid and/or the antibody, a method of treating or preventing a Borrelia infection and a method of immunizing a subject.

BACKGROUND OF THE INVENTION

Lyme borreliosis, or Lyme disease, is the most commonly reported tick-borne disease in Europe and North America. The disease is caused by infection with the arthropod-borne gram-negative-like spirochete, Borrelia burgdorferi sensu lato (B. burgdorferi s.l.), and can involve multiple organs or tissues, resulting in skin, cardiac, musculoskeletal and neurological disorders. In most countries, Lyme borreliosis is not a notifiable disease; therefore, exact data regarding annual incident rates are not available. In the United States, the causative agent is B. burgdorferi sensu stricto (B. burgdorferi s.s.) and Lyme borreliosis is localized to north-eastern, mid-Atlantic and upper north-central states. In 2010, a total of about 30,000 cases of Lyme borreliosis were reported to the US to the Centers for Disease Control and Prevention (CDC). An updated report by the CDC in 2013, which takes into account diagnostic data from other sources, estimates that the actual number of new cases per year in the United States is closer to 300,000 (http://www.cdc.gov/media/releases/2013/p0819-lyme-disease.html). In Europe, B. afzelii and B. garinii are the main causative agents of Lyme borreliosis, as well as B. burgdorferi s.s. and B. bavariensis, which contribute to a lesser extent depending on the geographic location. The prevalence of Lyme borreliosis varies considerably in different European countries with an overall increased prevalence from west to east. In much of Europe, the number of reported cases of Lyme borreliosis has increased since the early 1990s (e.g., the Czech Republic, Estonia, Lithuania; see Lyme borreliosis in Europe, WHO report of 2006), and the geographic distribution of cases has also expanded.

Borrelia belongs to the family Spirochaetaceae, which is subdivided into the medically important genera Treponema, Leptospira and Borrelia. B. burgdorferi s.l. is a spiral-shaped, vigorously motile gram-negative bacterium, about 10-20 μm long and 0.2-0.5 μm wide, that grows under microaerophilic conditions. The spirochetal cell wall consists of a cytoplasmic membrane surrounded by peptidoglycan and several flagella and then by a loosely-associated outer membrane.

Lyme borreliosis generally occurs in stages characterized by different clinical manifestations, with remissions and exacerbations. Stage 1, early infection, consists of a localized infection of the skin, followed within days or weeks by stage 2, disseminated infection, and months to years later by stage 3, persistent infection. However, the infection is variable; some patients have only localized infections of the skin, while others display only later manifestations of the illness, such as arthritis. Different clinical syndromes of Lyme borreliosis are also caused by infection with diverse B. burgdorferi s.l. species. B. burgdorferi s.s. more often causes joint manifestations (arthritis) and heart problems, B. afzelii causes mainly dermal symptoms (erythema migrans; EM and acrodermatitis chronica atrophicans; ACA), whereas B. garinii is implicated in most cases of neuroborreliosis.

Localized infection—The most common symptom of stage 1 of an infection is erythema migrans, which occurs in 70-80% of infected people. This skin lesion is often followed by flu-like symptoms, such as myalgia, arthralgia, headache and fever. These non-specific symptoms occur in 50% of patients with erythema migrans.

Disseminated infection—During stage 2, the bacteria move into the blood stream from the site of infection to distal tissues and organs. Neurological, cardiovascular and arthritic symptoms that occur in this stage include meningitis, cranial neuropathy and intermittent inflammatory arthritis.

Persistent infection—Stage 3 of the infection is chronic and occurs from months to years after the tick bite. The most common symptom in North America is rheumatoid arthritis, caused by an infection with B. burgdorferi s.s. Persistent infection of the central nervous system with B. garinii causes more severe neurological symptoms during stage 3, and a persistent infection of the skin with B. afzelii results in acrodermatitis chronica atrophicans.

In some risk groups, such as farmers, forestry workers, hikers, runners or vacationers, seroprevalence and disease incidence rates have increased, as well as in children under 15 years of age and adults between 39 and 59, without gender preference. This increased incidence of Lyme borreliosis is linked to changes in forest habitats as well as social factors. Environmental changes, such as forest fragmentation, have led to a sharp reduction of rodent predators such as foxes and birds of prey, which in turn has led to an increase in the mouse population, with a subsequent increase in the tick population. More recently, patchy reforestation has increased the number of deer and thus the number of ticks. Suburban sprawl and the increasing use of woodland areas for recreation such as camping and hiking has brought humans into greater contact with the larger number of tick Borrelia vectors. All of these factors together have contributed to a wider distribution of Borrelia and a higher incidence of Lyme borreliosis.

Antimicrobial agents are the principle method of treatment of Borrelia infection. The antibiotic used depends on the stage of the disease, symptoms, and the patient's allergies to medication. The length of the antibiotic course also depends on the stage of the disease and the severity of symptoms. Early Lyme borreliosis is typically treated with oral tetracyclines, such as doxycycline, and semi-synthetic penicillins, such as amoxicillin or penicillin V. Arthritic and neurological disorders are treated with high-dose intravenous penicillin G or ceftriaxone. Up to 30% of Lyme borreliosis patients do not display the early characteristic symptoms of infection with Borrelia, making diagnosis and treatment problematic. The antibiotic course can be long (up to several months) and sometimes ineffective and is thus debated in the Borrelia field, especially during later-stage disease. Even in the case of effective treatment of Borrelia, patients can be left with debilitating fatigue, pain, or neurological symptoms for years afterwards, which is referred to as post-treatment Lyme disease syndrome. In general, the use of antibiotics can have undesirable consequences, such as the development of resistance by the target micro-organisms. Finally, antibiotic therapy may effectively cure Lyme borreliosis, but provides no protection against subsequent infections.

A monovalent serotype 1-OspA-based vaccine (LYMErix™) was approved and marketed in the USA for the prevention of Lyme disease caused by Borrelia burgdorferi s.s., but the vaccine is no longer available. Furthermore, heterogeneity in OspA sequences across different serotypes in Europe and elsewhere precludes efficient protection with a vaccine based on OspA from only a single serotype.

Chimeric OspA molecules comprising the proximal portion from one OspA serotype, together with the distal portion form another OspA serotype, while retaining antigenic properties of both of the parent polypeptides, may be used in the prevention and treatment of Lyme disease or borreliosis (WO2011/143617, WO2011/143623).

Currently, there is no preventative medicament for Lyme borreliosis on the market and thus there is a need in the art for the development of such a medicament that can provide effective protection against Borrelia that are present in the USA, Europe and elsewhere, especially for the development of a medicament that can provide effective protection against several Borrelia serotypes simultaneously. The serotype 3 OspA-containing heterodimer, Lip-S4D1-S3hybD1, of the current invention improves on our previously-disclosed heterodimer, Lip-S4D1-S3D1 (see WO2014/006226) with regard to both ease of production and stimulation of specific antibodies as measured by antibody surface binding to serotype 3 Borrelia spirochetes. Additionally, the more specific quality of the immune response to the new heterodimer indicates that the potency is also superior in comparison to the previously-disclosed heterodimer.

SUMMARY OF THE INVENTION

The present invention relates to a polypeptide comprising a hybrid fragment of Borrelia outer surface protein A (OspA), a nucleic acid encoding the same, a vector which comprises such nucleic acid molecule, and a host cell comprising such vector. Furthermore, the invention provides a process for producing such polypeptide and a process for producing a cell which expresses such polypeptide. Moreover, the present invention provides antibodies specifically binding to such polypeptide, a hybridoma cell producing such antibodies, methods for producing such antibodies, a pharmaceutical composition comprising such polypeptide, nucleic acid molecule, vector or antibody, the use of such polypeptide, nucleic acid molecule, vector or antibody for the preparation of a medicament or a pharmaceutical composition (particularly for use as a vaccine or in a method of treating or preventing a Borrelia infection), methods for diagnosing an infection and methods for treating or preventing a Borrelia infection and methods of immunizing a subject. In particular, the invention relates to an improved polypeptide for immunization against serotype 3 Borrelia, in that the polypeptide is more favourable for purification and induces a more specific immune response to Borrelia as assessed by surface binding to Borrelia when compared with the serotype 3 OspA-containing construct disclosed in our previous application WO2014/006226.

Efforts to develop a subunit vaccine for prevention of Lyme borreliosis have been focused in large part on the use of borrelial outer surface protein A (OspA) as an antigen. The OspA protein is expressed by Borrelia only when it is in the gut of the tick vector. Thus, OspA antibodies produced by vaccination do not fight infection in the body, but rather enter the gut of the tick when it takes a blood meal. There, the antibodies neutralise the spirochetes and block the migration of bacteria from the midgut to the salivary glands of the tick, the route through which Borrelia enters the vertebrate host. Thus, OspA-specific antibodies prevent the transmission of Borrelia from the tick vector to the human host.

The lipidated form of OspA from B. burgdorferi s.s., strain ZS7, together with aluminium hydroxide was commercially developed as a vaccine against Borrelia (LYMErix™) by SmithKline Beecham, now GlaxoSmithKline (GSK) for the US market. Three doses of LYMErix™ over a period of one year were needed for optimal protection. After the first two doses, vaccine efficacy against Lyme borreliosis was 49%, and after the third dose, 76%. However, shortly after LYMErix™ was commercially available, it was withdrawn from the market in 2002. Reasons cited were matters of practical application of the vaccine, for example the need for booster injections every year or every other year, as well as the relatively high cost of this preventive approach compared with antibiotic treatment of early infection. In addition, there was a concern that LYMErix™ could trigger autoimmune reactions in a subgroup of the population due to sequence homology with a human protein, although this was never proven. In addition, cross-protection against other clinically important Borrelia species was not provided by this vaccine.

Accordingly, in one embodiment, it was an object of the present invention to provide an improved vaccine, e.g. for the prevention of Lyme borreliosis. Preferably, the vaccine is easily produced while being protective, safe and more effective than existing therapies and/or provides protection against more than one Borrelia species. Additionally, it is well-known in the art that different patients respond differently to different vaccine compositions. Therefore, in any case, it would be a distinct advantage to have alternative vaccines available as additional options for vaccinations against Borrelia.

The problem underlying the present invention is solved by a polypeptide comprising a hybrid C-terminal OspA fragment, wherein the hybrid fragment consists of a C-terminal domain of an OspA protein of Borrelia that is comprised of a fragment derived from an OspA protein of a Borrelia strain different than B. garinii, strain PBr, and a second fragment of OspA from B. garinii, strain PBr, and differs from the corresponding wild-type sequence at least by the introduction of at least one disulfide bond.

Surprisingly, the introduction of said hybrid C-terminal OspA fragment comprising a sequence from B. valaisiana, strain VS116, fused with a sequence from serotype 3 OspA (B. garinii, strain PBr), with an introduced disulfide bond, resulted in a heterodimer protein (Lip-S4D1-S3hybD1) that was easier to purify and stimulated a more specific immune response to serotype 3 OspA than the heterodimer Lip-S4D1-S3D1 of our previous invention (WO2014/006226). As shown in the Examples, the serotype 3 hybrid OspA C-terminal fragment of the present invention has a predicted electrostatic potential isocontour that is more similar to other OspA fragments from other serotypes than the serotype 3 OspA fragment. Furthermore, the serotype 3 hybrid OspA C-terminal fragment-containing heterodimer (Lip-S4D1-S3hybD1) was easier to purify, requiring less steps to get a much higher yield than the Lip-S4D1-S3D1 heterodimer of the previous invention. Additionally, although the observed antibody titers were similar, the antibodies stimulated by the improved heterodimer combination vaccine bound more specifically to Borrelia expressing serotype 3 OspA compared with the heterodimer combination vaccine of the previous invention. Finally, the in vivo protective capacity of the improved heterodimer combination vaccine was high against the four Borrelia serotypes tested.

DETAILED DESCRIPTION OF THE INVENTION

Accordingly, in a first aspect, the present invention relates to a polypeptide comprising a hybrid C-terminal fragment of an outer surface protein A (OspA), wherein said hybrid C-terminal OspA fragment consists of a C-terminal domain fusion of two different OspA of Borrelia strains and differs from the corresponding wild-type fragment at least by the introduction of at least one disulfide bond, e.g. a cystine. In particular, the hybrid C-terminal OspA fragment consists of a fusion of amino acids from the OspA proteins from a strain different to B. garinii, strain PBr, e.g., B. valaisiana, strain VS116, or B. spielmanii; with amino acids from the OspA protein of B. garinii, strain PBr, with the introduction of at least one disulfide bond (cystine). Specifically, the polypeptide comprises a hybrid C-terminal OspA (outer surface protein A of Borrelia) fragment, wherein the hybrid C-terminal OspA fragment consists, from the N- to C-terminal direction, of i) a first OspA portion consisting of amino acids 125-176 or amino acids 126-175 of OspA from a Borrelia strain that is not the corresponding fragment of B. garinii, strain PBr, with SEQ ID NO: 8, and ii) a second OspA portion consisting of amino acids 177-274 or amino acids 176-274, (but most preferably amino acids 177-274), of OspA from B. garinii, strain PBr (SEQ ID NO: 8), wherein the second OspA portion is mutant and cystine-stabilized in that it differs from the corresponding wild-type sequence at least by the substitution of the wild-type amino acid at position 182 of SEQ ID NO: 8 by a cysteine and by the substitution of the wild-type amino acid at position 269 of SEQ ID NO: 8 by a cysteine and wherein a disulfide bond between the cysteine at position 182 and the cysteine at position 269 of said second OspA fragment is present; and wherein the numbering of the amino acids and of the cysteine substitutions is according to the numbering of corresponding amino acids of the full length OspA of B. burgdorferi s.s., strain B31 (SEQ ID NO: 5).

Alternatively, the polypeptide comprises a hybrid C-terminal OspA fragment, wherein the hybrid C-terminal OspA fragment consists, from the N- to C-terminal direction, of

-   -   i) a first OspA fragment (also referred to as a first OspA         portion) consisting of amino acids 125-176 or amino acids         126-175 of OspA from a Borrelia strain that is not the         corresponding fragment of B. garinii, strain PBr, with SEQ ID         NO: 8, and     -   ii) a second OspA fragment (also referred to as a second OspA         portion) consisting of amino acids 177-274 of OspA from B.         garinii, strain PBr (SEQ ID NO: 8), wherein the second OspA         fragment differs from the corresponding wild-type sequence by         the substitution of the wild-type amino acid at position 182 of         SEQ ID NO: 8 by a cysteine and by the substitution of the         wild-type amino acid at position 269 of SEQ ID NO: 8 and wherein         a disulfide bond between the cysteine at position 182 and the         cysteine at position 269 of said second OspA fragment is         present; and

wherein the numbering of the cysteine substitutions is according to the numbering of corresponding amino acids of the full length OspA of B. burgdorferi s.s., strain B31 (SEQ ID NO: 5).

It has been found that a polypeptide according to the invention can be easily and effectively produced (see Example 2). Its production resulted in an increased yield in comparison to known products (see Example 2) Immunization with a polypeptide according to the invention produced higher levels of antibodies specific to the OspA protein in its native form and is thus an improved vaccine (see Example 3). Moreover, it provided protection against in vivo Borrelia challenge (see Example 4).

Borrelia is a genus of bacteria of the spirochete phylum. It causes borreliosis, a zoonotic, vector-borne disease transmitted primarily by ticks and some by lice, depending on the species. At present there are 36 known species of Borrelia. Of the 36 known species of Borrelia, 13 of these species are known to cause Lyme disease or borreliosis and are transmitted by ticks. The major Borrelia species causing Lyme disease are Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii. The term B. burgdorferi s.l. encompasses at least 13 Borrelia species (Table A-1). These species occur in different geographic regions, and live in nature in enzootic cycles involving ticks of the Ixodes ricinus complex (also called Ixodes persulcatus complex) and a wide range of animal hosts. Four Borrelia species are responsible for the majority of infections in humans: B. burgdorferi s.s., B. afzelii, B. bavariensis and B. garinii. Three other species, B. lusitaniae, B. bissettii and B. spielmanii, have occasionally been detected in humans, but their role in Lyme borreliosis is uncertain at present. New species of Borrelia are still being identified.

TABLE A-1 Pathogenic species (4) Principal tick vector Location Borrelia burgdorferi Ixodes scapularis Northeastern/ north-central US (Borrelia burgdorferi s.s.) Ixodes pacificus Western US Ixodes ricinus Europe Ixodes persulcatus Asia Borrelia garinii Ixodes ricinus Europe Ixodes persulcatus Asia Borrelia afzelii Ixodes ricinus Europe Ixodes persulcatus Asia Borrelia bavariensis Ixodes ricinus Europe Ixodes persulcatus Asia Minimally pathogenic or non- pathogenic species (9) Principal tick vector Location Borrelia andersonii Ixodes dentatus Eastern US Borrelia bissettii Ixodes spinipalpis Western US Ixodes pacificus Europe Ixodes ricinus Borrelia valaisiana Ixodes ricinus Europe and Asia Ixodes columnae Borrelia lusitaniae Ixodes ricinus Europe Borrelia spielmanii Ixodes ricinus Europe Borrelia japonica Ixodes ovatus Japan Borrelia tanukii Ixodes tanuki Japan Borrelia turdi Ixodes turdus Japan Borrelia sinica Ixodes persulcatus China

As detailed above, Borrelia outer surface protein A (OspA) is an abundant immunogenic lipoprotein of Borrelia of particular interest because of its potential as a vaccine candidate. OspA of B. burgdorferi s.l. is a basic lipoprotein that has a molecular mass of approximately 30 kDa and is encoded on a linear plasmid. An important aspect of the OspA protein is its N-terminal lipidation; that is, fatty acids with a chain length of between C14 and C19 with or without double bonds are attached to the N-terminal cysteine residue, a feature that enhances the immunogenicity of the OspA protein. It has been shown that poorly-immunogenic synthetic peptides induce stronger antibody responses when lipidated; for example, when covalently coupled to Pam3Cys (Bessler and Jung, Research Immunology (1992) 143:548-552), a fatty acid substitution found at the amino terminus of many bacterial lipoproteins that are synthesized with a signal sequence specifying lipid attachment. Additionally, the Pam3Cys moiety was shown to enhance immune responses to OspA in mice, partially through its interaction with TLR-2/1 (Yoder, et al. (2003) Infection and Immunity 71:3894-3900). Therefore, lipidation of a C-terminal fragment of OspA would be expected to enhance the immunogenicity and protective capacity of the fragment.

Analysis of isolates of B. burgdorferi s.l. obtained in North America and Europe has revealed that OspA has antigenic variability and that several distinct groups can be defined based on serology. Anti-OspA mAbs which bind to specific N- and C-terminal antigenic determinants have been reported. X-ray crystallography and NMR analysis have been used to identify immunologically important hypervariable domains in OspA and have mapped the LA-2 epitope to C-terminal amino acids 203-257 (Ding et al., Mol. Biol. 302: 1153-64, 2000). Previous studies have shown that the production of antibodies against the C-terminal epitope LA-2 correlates with protective immunity after vaccination with OspA (Van Hoecke et al. Vaccine (1996) 14(17-18):1620-6 and Steere et al., N Engl J Med (1998) 339:209-215). Antibodies to LA-2 were shown to block the transmission of Borrelia from tick to host (Golde et al., Infect Immun (1997) 65(3):882-889). These studies suggested that the C-terminal portion of the OspA protein may be sufficient for inducing protective immunity. It should be noted that the sequence of the C-terminal portion of OspA is less highly-conserved between Borrelia serotypes than is the N-terminal portion (see FIG. 1 ).

Based on information from the studies outlined above, along with others, truncated forms of OspA comprising the C-terminal portion (also referred to herein as “OspA fragment” or “monomer”) were used in the previous invention (WO2014/006226). The truncated forms of OspA proved to be less protective than the full-length OspA protein. Surprisingly, however, it was found in the course of the previous invention that the introduction of a disulfide bond in the truncated form (also referred to herein as “mutant OspA fragment”, “cystine-stabilized OspA fragment”, or “mutant fragment” or “cystine-stabilized fragment”) overcomes this disadvantage. While not being limited to a specific mechanism, it is thought that improved protection is due to increased stability of the OspA fragment, as shown in assays measuring thermal stability.

In accordance with the previous invention, the mutant OspA fragment (in the present invention also referred to as second OspA fragment) may be derived from any Borrelia species; however, due to their prevalence in the medical field, particularly for humans, B. burgdorferi s.s., B. afzelii, B. bavariensis and B. garinii are preferred. In accordance with the present invention, the first OspA portion is from any Borrelia strain except B. garinii, strain PBr and the second portion is from B. garinii, strain PBr. Therefore, the hybrid OspA fragment is derived from a fusion of amino acids from the OspA of B. garinii, strain PBr with amino acids from the OspA from any Borrelia species except B. garinii, strain PBr (and therefore an amino acid sequence different from that amino acids from the OspA from B. garinii, strain PBr), particularly from B. burgdorferi s.s., B. afzelii, and B. bavariensis, especially B. valaisiana, strain VS116. The first OspA portion consists of amino acids 125-176 or amino acids 126-175 of OspA from a Borrelia strain that is not the corresponding fragment of B. garinii, strain PBr, with SEQ ID NO: 8, and the second OspA portion consists of amino acids 176-274 or most preferably amino acids 177-274 of OspA from B. garinii, strain PBr (SEQ ID NO: 8), wherein the second OspA portion is mutant and cystine-stabilized in that it differs from the corresponding wild-type sequence at least by the substitution of the wild-type amino acid at position 182 of SEQ ID NO: 8 by a cysteine and by the substitution of the wild-type amino acid at position 269 of SEQ ID NO: 8 by a cysteine and wherein a disulfide bond between the cysteine at position 182 and the cysteine at position 269 of said second OspA fragment is present; and wherein the numbering of the amino acids and of the cysteine substitutions is according to the numbering of corresponding amino acids of the full length OspA of B. burgdorferi s.s., strain B31 (SEQ ID NO: 5). However, further mutations relative to the wild-type portions may be present in the first and second portions according to the invention (see also below). In a preferred embodiment the above substitutions with cysteine at positions 182 and 269 are the only mutations relative to the wild-type. Alternatively, the cystine-stabilized amino acids 177-274 from Borrelia garinii, strain PBr differs therefrom by the substitution of the threonine residue at amino acid 233 of wild-type OspA of Borrelia garinii, strain PBr, with a proline residue only.

Preferred examples of polypeptides comprise

-   -   a hybrid C-terminal OspA fragment consisting of amino acids         125-176 from B. valaisiana, strain VS116, and the         cystine-stabilized amino acids 177-274 from Borrelia garinii,         strain PBr (SEQ ID NO: 1), or     -   a hybrid C-terminal OspA fragment consisting of amino acids         126-175 from B. spielmanii and the cystine-stabilized amino         acids 177-274 from Borrelia garinii, strain PBr (SEQ ID NO: 51).

Therefore, in a preferred embodiment of the invention, the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 1. Alternatively, the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 51.

In one embodiment of the present invention, the second OspA portion is identical to the cystine-stabilized amino acids 177-274 from Borrelia garinii, strain PBr, but differs therefrom by the substitution of the threonine residue at amino acid 233 of wild-type OspA of Borrelia garinii, strain PBr, with a proline residue (SEQ ID NO: 7).

The four Borrelia species B. burdorferi s.s., B. afzelii, B. bavariensis and B. garinii can be further classified according to their OspA serotypes, which have been determined by analysis with monoclonal antibodies specific to the respective OspA protein. Serotypes 1-7, which account for the majority of human Borrelia infections, along with their rates of prevalence, are shown in Table A-2 below.

TABLE A-2 Serotype designation and prevalence of B. burdorferi s.s., B. afzelii, B. bavariensis and B. garinii. Borrelia isolated from human cerebrospinal fluid or skin or from tick vectors were serotyped by probing whole-cell lysates with mouse monoclonal antibodies, each specific to a particular epitope of OspA (as described by Wilske et al., J. of Clin Microbiol (1993) 31(2): 340-350 and presented by Baxter Bioscience at “Climate change effect on ticks and tick-borne diseases”, Brussels, 6 Feb. 2009). OspA serotype defined Prevalence in Strain source Borrelia sp. by mAb testing human disease for sequence Seq ID No: B. burgdorferi s.s. 1  11% B31  5 B. afzelii 2  63% K78  6 B. garinii 3 1.5% PBr 7, 8 B. bavariensis 4   4% PBi  9 B. garinii 5   6% PHei 10 B. garinii 6  13% DK29 11 B. garinii 7 0.5% T25 12

The structure of the OspA protein from B. burgdorferi s.s. strain B31 was determined by Li et al. (Proc Natl Acad Sci (1997) 94:3584-3589). It is composed of N-terminal (α-strands 1 to 4) and central β-sheets (α-strands 5 to 14n [N-terminal part]), barrel sheet 1 (α-strands 14c [C-terminal part] to 16), barrel sheet 2 (α-strands 17 to 21) and a C-terminal α-helix. The term “C-terminal OspA fragment” or “OspA C-terminal domain” or “C-terminal domain” or “wild-type fragment” or “C-terminal portion” with respect to OspA as used throughout the present specification shall mean the C-terminal amino acid sequence of OspA, i.e., OspA lacking at least the N-terminal β-sheet (including α-strands 1 to 4). In OspA from B. burgdorferi s.s. strain B31, the N-terminal sheet consists of amino acids 17 to 70 (following post-translational cleavage of the 16 amino acid long lipidation signal peptide).

The C-terminal OspA fragment of the current invention may also include a lipidation signal sequence at the N-terminus, e.g., the lipidation signal sequence of amino acids 1 to 16 of OspA (SEQ ID NO: 13) or OspB (SEQ ID NO: 14) from B. burgdorferi s.s. strain B31, a lipidation signal sequence from E. coli, referred to herein as the “lpp lipidation signal” (SEQ ID NO: 15), or any other signal sequence, e.g., as defined below.

Lipidation of a protein with an N-terminal lipidation signal sequence, such as those present on a nascent OspA polypeptide, occurs in the E. coli expression vector by the step-wise action of the enzymes diacylglyceryl transferase, signal peptidase II and transacylase, respectively. The first step is the transfer of a diacylglyceride to the cysteine sulfhydryl group of the unmodified prolipoprotein, followed by the cleavage of the signal peptide by signal peptidase II and, finally, the acylation of the □-amino group of the N-terminal cysteine of the apolipoprotein. The result is the placement of one lipid and a glycerol group with two further lipids attached on the N-terminal cysteine residue of the polypeptide. The lipidation signal sequence, which is cleaved off during lipidation, is not present in the final polypeptide sequence.

Polypeptides are biological molecules of chains of amino acid monomers linked by peptide (amide) bonds. The covalent chemical bonds are formed when the carboxyl group of one amino acid reacts with the amino group of another. Polypeptides of the present invention are continuous and unbranched amino acid chains. The polypeptide of the present invention comprises a cystine bond. According to the current invention, the mutant OspA fragment may be a lipidated protein, also lipoprotein, wherein the lipid moieties, along with the glycerol group, is also referred to as “Lip”. According to the invention, Lip comprises one to three lipids such as C₁₄₋₂₀ alkyl and/or C₁₄₋₂₀ alkenyl attached to a glycerol and an amino group of the N-terminal cysteine of the polypeptide of the invention, or preferably wherein Lip is a moiety of formula (I) below,

in which one of R₁, R₂ or R₃ is C₁₄-C₂₀ alkyl or alkenyl, and each of the others, independently, is C₁₄-C₂₀ alkyl or C₁₄-C₂₀ alkenyl, and X is an amino acid sequence attached to the cysteine residue shown in Formula (I). More preferably, Lip plus the N-terminal cysteine of the polypeptide is N-palmitoyl-S-(2RS)-2,3-bis-(palmitoyloxy) propyl cysteine (referred to herein as “Pam3Cys”) and is connected via the carbonyl C of the cysteine to said amino acid sequence of the invention. In Formula (I) above, R₁, R₂ and R₃ would be palmitoyl moieties and X is an amino acid sequence attached to the cysteine residue.

In accordance with the current invention, the C-terminal domain of an OspA may lack at least the N-terminal domain homologous to amino acids 17 to 70 of OspA from B. burgdorferi s.s., strain B31. Additionally, the OspA C-terminal domain according to the present invention may also lack further portions of the central sheet as defined by Li and co-workers (Li et al., supra), particularly further strands such as the amino acid portions from amino acid 17 to 82, 93, 105, 118 or 119, preferably 17 to 129, more preferably 1 to 124, 1 to 125, 1 to 129 or 1 to 130 of any Borrelia, particularly B. burgdorferi s.s., strain B31, or homologous portions of an OspA protein from a Borrelia sp. other than B. burgdorferi s.s., strain B31.

In the context of the present invention, the OspA C-terminal domain is also referred to as “OspA fragment” or “fragment of OspA”.

The “mutant C-terminal OspA fragment” or “mutant fragment” or “mutant OspA fragment” in the context of the polypeptide of the present invention and as used throughout the present specification shall mean the OspA C-terminal fragment, as defined above and herein, which differs from the wild-type fragment at least by at least two introduced cysteines that can form a disulfide bond; i.e., a cystine. Without being bound to that theory, it is assumed that the disulfide bond stabilizes the fragment in a conformation conducive to the induction of antibody binding. The fold of the wild-type C-terminal fragment of OspA shows reduced temperature stability in comparison to the full-length protein (Koide et al., Structure-based Design of a Second-generation Lyme Disease Vaccine Based on a C-terminal Fragment of Borrelia burgdorferi OspA, J. Mol. Biol. (2005) 350:290-299). For the present invention, the sequence of the C-terminal domain of the B. burgdorferi s.s., strain B31 OspA has been in silico analyzed to determine positions for introduced disulfide bridges that may enhance the stability of the fold of this C-terminal domain. The results of the analysis have been transferred to homologous OspA fragments of other Borrelia species with the assumption that the fold is conserved across species.

The “hybrid C-terminal OspA fragment” or “hybrid fragment” or “hybrid OspA fragment” in the context of the polypeptide of the present invention and as used throughout the present specification shall mean the OspA C-terminal fragment, as defined above and herein, which differs from the wild-type fragment in that

-   -   a) the hybrid C-terminal OspA fragment consists of a fusion of         amino acids from a first OspA protein from a strain different         to B. garinii, strain PBr, e.g. B. valaisiana, strain VS116,         or B. spielmanii (referred to as first OspA portion); with amino         acids from a second OspA protein of B. garinii, strain PBr, with         the introduction of at least one disulfide bond (cystine)         (referred to as second OspA portion) and optionally one or more         further mutations; and     -   b) the at least two introduced cysteines in the hybrid         C-terminal OspA fragment form a disulfide bond, i.e. a cystine,         and wherein said introduced cysteines are introduced as         described herein and in accordance with the teaching of         WO2014/006226; and wherein the the hybrid C-terminal OspA         fragment results in a naturally folded fragment as measured e.g.         by the efficiency of binding of antibodies resulting from the         vaccination by this hybrid C-terminal OspA fragment in e.g. mice         to Borrelia serotype 3 antigen, e.g. by testing the binding of         said antibodies (obtained after three immunizations) to the         surface of serotype 3 Borrelia by flow cytometry, e.g. as         described in the Examples.

Typically, the disulfide bond may be introduced by the introduction of one or more, preferably two, cysteine residues, wherein a disulfide bond (S-S bridge) is formed between the thiol groups of two cysteine residues forming the amino acid cystine. Only one cysteine residue need be introduced if a disulfide bond is formed with a cysteine residue present in the wild-type OspA fragment. The two cysteines are introduced by amino acid substitution. Substitutions are a) at position 182 of the amino acid of the relevant wild-type OspA amino acid sequence by a cysteine and b) at position 269 of the amino acid of the relevant wild-type OspA amino acid sequence by a cysteine and wherein a disulfide bond between the cysteine at position 182 and the cysteine at position 269 of said OspA fragment is present forming thus a cystine. The numbering of the cysteine substitutions is according to the numbering of corresponding amino acids of the full length OspA of B. burgdorferi s.s., strain B31 (SEQ ID NO: 5).

The mutant or hybrid OspA fragment may also comprise further mutations relative to the wild-type. As detailed above, the structure and surface domain of OspA are known in the art. Accordingly, the mutant fragment may comprise further mutations, particularly at sites not on the surface of the protein and/or not involved in the immune response and, therefore not impacting antigenic capacity. These can include one or more amino acid deletion(s), particularly small (e.g., up to 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 amino acids) deletions, one or more amino acid addition(s) (particularly C- or N-terminally), one or more amino acid substitution(s), particularly one or more conservative amino acid substitutions. Preferably, the number of further muations in the first and second portion relative to the respective wild-type is at most 10, 9, 8, 7, 6, 5, 4, more preferably 3 or 2, especially 1. More preferably the further mutation(s) is/are in the second OspA portion only. Preferred mutations are substitutions, especially conservative substitutions. Examples of conservative amino acid substitutions include, but are not limited to, those listed below:

Ala Ser Leu Ile; Val Arg Lys Lys Arg; Gln; Asn Asn Gln; His Met Leu; Ile Asp Glu Phe Met; Leu; Tyr Cys Ser Ser Thr Gln Asn Thr Ser Glu Asp Trp Tyr His Asn; Gln Tyr Trp; Phe Ile Leu, Val Val Ile; Leu

Preferred mutations include changes in selected portions of the fragment, for example, wherein the sequence with sequence similarity to human leukocyte function-associated antigen (hLFA-1), which exists in B. burgdorferi s.s. is modified, for example, replaced by a homologous sequence from an OspA protein from another Borrelia sp. The rationale for this modification is to reduce the risk for inducing immunological cross-reaction with human proteins. Another preferred mutation is the substitution of a proline for the threonine at position 233 in the OspA polypeptide sequence from B. garinii, strain PBr (SEQ ID NO: 8). Also possible is the addition of a signal sequence for lipidation in the final, or an intermediate, fragment, or the addition of a marker protein (e.g., for identification or purification).

In some embodiments, the mutant or hybrid OspA fragment has an amino acid sequence that has 60%, preferably at least 70%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, even more preferably at least 95% sequence identity to the wild-type fragment. In another embodiment, the sequence differs by at most 10%, at most 9%, at most 8%, at most 7%, at most 6%, 5%, 4%, 3%, 2%, most preferably at most 1%, due to a sequence addition, deletion or substitution.

Identity, as known in the art and as used herein, is the relationship between two or more polypeptide sequences, as determined by comparing the sequences. In the art, identity also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. Identity can be readily calculated. While a number of methods exist to measure identity between two polynucleotides or two polypeptide sequences, the term is well known to skilled artisans (e.g. Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987). Preferred methods to determine identity are designed to give the largest match between the sequences tested. Methods to determine identity are codified in computer programs. Preferred computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package (Devereux, J. et al., 1984), BLASTP, BLASTN, and FASTA (Altschul, S. et al., 1990).

In contrast to the mutant or hybrid OspA fragment, the “wild-type fragment” or “wild-type OspA fragment” in the context of the present invention relates to a fragment of a naturally-occurring OspA of Borrelia. The wild-type fragment is obtained by N-terminal deletions, but it does not comprise internal deletions (except from signal sequences as detailed herein) or mutations. In relation to the hybrid and mutant OspA fragment, the wild-type fragment consists of an identical part of the OspA (identical length and same strain of OspA, etc.) and differs only in the alteration(s) detailed above.

The Polypeptides of the Invention

A polypeptide is a single linear polymer of amino acids linked by peptide bonds, in some cases also by disulfide bonds. In accordance with the present invention, the polypeptide may also comprise one or more posttranslational modifications; i.e., an attached biochemical functional group, such as an attached acetate, phosphate, lipid or carbohydrate, preferably a lipid or lipids attached to the N-terminal cysteine along with a glycerol, more preferably 1 to 3 C₁₄-C₂₀ alkyl or alkenyl moieties, even more preferably 1 to 3 palmitoyl groups, most preferably three palmitoyl groups (Pam₃).

The polypeptide of the present invention is as defined above and comprises or consists of a hybrid C-terminal OspA fragment, wherein the hybrid C-terminal OspA fragment consists, from the N- to C-terminal direction, of

-   -   i) a first OspA portion consisting of amino acids of a first         C-terminal part of OspA from a Borrelia strain that is not the         corresponding fragment of B. garinii, strain PBr, with SEQ ID         NO: 8, and starts at a position around 125 or 126 and ends at         position 175 or 176; and     -   ii) a second OspA portion consisting of a second C-terminal part         of OspA that continuously follows the first C-terminal part         (e.g. if the first C-terminal part ends at position 175, the         second C-terminal part continues at position 176; or if the         first C-terminal part ends at positions 176, the second         C-terminal part continues at positions 177 and so forth, however         it may also be that one or two or more up to 10 amino acids may         be deleted in the fusion area of the 2 C-terminal parts, e.g.         and most preferably if the first C-terminal part ends at         position 175, the second C-terminal part continues at position         177), wherein the second OspA fragment differs from the         corresponding wild-type sequence by the substitution of the         wild-type amino acid at position 182 of SEQ ID NO: 8 by a         cysteine and by the substitution of the wild-type amino acid at         position 269 of SEQ ID NO: 8 by a cysteine and wherein a         disulfide bond between the cysteine at position 182 and the         cysteine at position 269 of said second OspA fragment is present         forming a cysteine (also referred to as “cystine-stablized OspA         fragment”); and     -   wherein the numbering of the amino acids and of the cysteine         substitutions is according to the numbering of corresponding         amino acids of the full length OspA of B. burgdorferi s.s.,         strain B31 (SEQ ID NO: 5).

In a further embodiment of the present invention, the polypeptide comprises or consists of the hybrid C-terminal OspA fragment consisting of amino acids 125-176 from B. valaisiana, strain VS116, and the cystine-stabilized amino acids 177-274 from Borrelia garinii, strain PBr (SEQ ID NO: 1).

In a further embodiment of the present invention, the polypeptide comprises or consists of the hybrid C-terminal OspA fragment consisting of amino acids 126-175 from B. spielmanii and the cystine-stabilized amino acids 177-274 from Borrelia garinii, strain PBr (SEQ ID NO: 51).

In a further embodiment of the present invention, the polypeptide is as defined herein, and wherein the second OspA portion is identical to the cystine-stabilized amino acids 177-274 from Borrelia garinii, strain PBr, but differs therefrom only by the substitution of the threonine residue at amino acid 233 of wild-type OspA of Borrelia garinii, strain PBr, with a proline residue.

The polypeptides of the invention as defined above and further as defined herein provide for antibody titers in immunized animals, wherein said antibodies show improved binding to their respective antigens in situ when compared to relevant prior art polypeptides (e.g. as defined in WO2014/006226). Preferably, a polypeptide comprising the hybrid C-terminal OspA fragment of the invention effects at least a 1.5-fold increase, preferably at least a 2-fold increase, more preferably at least a 3-fold increase, even more preferably at least a 4-fold increase, even more preferably at least a 5-fold increase, most preferably at least a 10-fold increase in fluorescence intensity, measured by flow cytometry and illicited by antibodies raised after three immunizations with the polypeptide in mice by binding to the surface of serotype 3 Borrelia, in comparison to the fluorescence intensity illicited by antibodies raised to a polypeptide comprising a C-terminal domain of an OspA protein of Borrelia which differs from the corresponding wild-type OspA sequence by at least the addition of at least one cysteine bond, more preferably the Lip-S4D1-S3D1 heterodimer protein as defined by SEQ ID NO: 31, particularly wherein the increase may be determined as described in Example 3.

Furthermore, in a further embodiment of the present invention, the polypeptide of the invention as defined above and herein can be produced in higher yields with standard processes and require less purification steps when compared to relevant prior art polypeptides (e.g. as defined in WO2014/006226). Preferably, a polypeptide comprising the hybrid C-terminal OspA fragment of the invention shows at least a 1.5-fold increase, preferably at least a 2-fold increase, more preferably at least a 3-fold increase, even more preferably at least a 4-fold increase, even more preferably at least a 5-fold increase, most preferably at least a 10-fold increase in production yield, measured in milligrams per gram biomass, in comparison to a polypeptide comprising a C-terminal domain of an OspA protein of Borrelia which differs from the corresponding wild-type OspA sequence by at least the addition of at least one cysteine bond, more preferably the Lip-S4D1-S3D1 heterodimer protein as defined by SEQ ID NO: 31, particularly wherein the increase may be determined as described in Example 2.

Preferably, a polypeptide comprising the hybrid C-terminal OspA fragment of the invention requires less steps for purification than a polypeptide comprising a C-terminal domain of an OspA protein of Borrelia which differs from the corresponding wild-type OspA sequence by at least the addition of at least one cysteine bond, more preferably the Lip-S4D1-S3D1 heterodimer protein as defined by SEQ ID NO: 31; more specifically, requires at least one less chromatography step.

In a further embodiment of the present invention, the polypeptide comprises a) a hybrid C-terminal OspA fragment as defined above and herein, and b) a mutant OspA fragment.

In a further embodiment of the present invention, the polypeptide of the invention comprises a) a hybrid C-terminal OspA fragment as defined herein, and b) a second OspA fragment, wherein said OspA fragment is C-terminal in that it consists of a C-terminal domain of an OspA protein of Borrelia and is mutant and cystine-stabilized in that it differs from the corresponding wild-type OspA sequence at least by the substitution of the amino acid at position 182 of the wild-type sequence by a cysteine and by the substitution of the amino acid at position 269 of the wild-type sequence by a cysteine and wherein a disulfide bond between the cysteine at position 182 and the cysteine at position 269 of said OspA fragment is present; and furthermore wherein said mutant OspA fragment starts at position 123, 124, or 125 and ends at position 273 or 274; and furthermore wherein the numbering of the amino acids and of the cysteine substitutions is according to the numbering of corresponding amino acids of the full length OspA of B. burgdorferi s.s., strain B31 (SEQ ID NO: 5). In one embodiment of the present invention, the second OspA fragment may be any of the mutant C-terminal fragments of OspA as defined above, e.g. in the context of the previous invention (WO2014/006226).

According to the present invention, said mutant OspA fragment and hybrid fragment of the invention do not comprise (i) the N-terminal sheet as defined above and (ii) optionally one or more further strands of the central sheet as defined above. However, the polypeptide may comprise one or more functional sequences such as a signal sequence, e.g., a lipidation signal sequence or a posttranslational modification, such as lipidation.

In a further embodiment of the present invention, the polypeptide of the present invention consists of (i) one or more mutant OspA fragments, wherein at least one is a hybrid C-terminal OspA fragment according to the present invention, optionally joined by linkers, e.g., as defined below or one or more mutant OspA fragments and a serotype 3 hybrid OspA C-terminal fragment and (ii) optionally one or more amino acids heterologous to OspA, particularly a signal sequence and (iii) optionally a posttranslational modification, such as lipidation.

Thus, in a further embodiment of the present invention, the polypeptide of the invention as defined above and herein additionally may comprise:

-   -   i) a polypeptide that is lipidated or wherein the polypeptide         comprises a lipidation signal, preferably the E. coli-derived         1pp lipidation signal MKATKLVLGAVILGSTLLAG (SEQ ID NO: 15);         and/or     -   ii) a polypeptide that comprises a lipidation site peptide lead         by an N-terminal cysteine residue as a site for lipidation,         preferably CSS; and/or     -   iii) a polypeptide that comprises a linker between the hybrid         C-terminal OspA fragment and the second cysteine-stabilized OspA         fragment, particularly wherein said linker comprises e.g.         GTSDKNNGSGSKEKNKDGKYS (SEQ ID NO: 16).

In one embodiment of the present invention, the second C-terminal OspA fragment is a hybrid C-terminal fragment of OspA according to the present invention.

In a further embodiment of the present invention, the polypeptide comprises or consists of the heterodimer of Lip-S4D1-S3hybD1 (SEQ ID NO: 27). Therefore, in a further aspect, the present invention relates to a polypeptide comprising or consisting of an amino acid sequence of SEQ ID NO: 27 (heterodimer of Lip-S4D1-S3hybD1).

The polypeptide of the present invention has protective capacity. As detailed above, the introduction of a disulfide bond into the hybrid and hybrid/mutant OspA fragment but also the hybrid nature of the OspA fragment of the invention increases the protective capacity of the polypeptide relative to a polypeptide comprising the respective fragment without the disulfide bond(s) and hybrid nature of the OspA fragment. In some embodiments, the protective capacity is increased by at least 10%, more preferably by at least 20%, more preferably by at least 30%, more preferably by at least 40%, more preferably by at least 50%, more preferably by at least 60%, more preferably by at least 70%, more preferably by at least 80%, even more preferably by at least 90% relative to a polypeptide comprising the respective fragment without the disulfide bond(s) and hybrid nature of the OspA fragment.

The term protective capacity describes the ability to protect a subject against a Borrelia infection. With respect to the polypeptide of the invention, protective capacity relates to the ability of the polypeptide to induce an immune response that protects a subject against a Borrelia infection. Protective capacity can be tested by administering to a subject the polypeptide in a manner to induce an immune reaction against the polypeptide. Thereafter, the subject may be challenged with Borrelia. The subject's reaction to the infection is monitored. Particularly, the presence of Borrelia in the subject may be determined. For example, the polypeptide is protective if Borrelia cannot be detected in the subject. The presence of Borrelia can be determined by detecting Borrelia-specific nucleic acids (e.g., by PCR) or Borrelia-specific antibodies (e.g., by ELISA or Western blot) or by detecting Borrelia itself (e.g., culturing organs or tissues in growth medium and verifying the presence of Borrelia by microscopy). In particular, the protective capacity (“pc”), reported as a percentage, for a particular dose is defined as follows:

pc(%)=[(number of total tested subjects−number of Borrelia-infected subjects)/number of total tested subjects]×100

Differences in protective capacity (Δpc) may be determined by, e.g. comparing the protective capacity (pc) of a mutant OspA fragment with a disulfide bond(s) (pc [with bond]) to the protective capacity of an OspA fragment without a disulfide bond(s) (pc [w/o bond]). In accordance with the present invention, the polypeptides to be compared differ only in the introduction of at least one disulfide bond. The change in protective capacity (Δpc) by the introduction of the disulfide bond(s) is determined as follows:

Δpc=(pc[sample]−pc[control])

e.g. Δpc=(pc[with bond]−pc[w/o bond])

If Δpc is greater than zero (>0), assuming all other parameters (e.g., dose and assay) are the same, then the protective capacity of the sample (e.g. the mutant OspA fragment with a disulfide bond(s)) is better than the protective capacity of the control (e.g. the OspA fragment without a disulfide bond(s)). Conversely, if Δpc is less than zero (<0) and assuming all other parameters (e.g., dose and assay) are the same, then the protective capacity of the sample (e.g. the mutant OspA fragment with a disulfide bond(s)) is less than the protective capacity of the comparison (e.g., the OspA fragment without a disulfide bond(s)).

Preferably, the polypeptide of the present invention is assessed for its protective capacity by an in vivo challenge assay wherein mice immunized with the polypeptide of the invention or with a placebo control are challenged with Borrelia introduced into the immunized subjects with a hypodermic needle (Needle Challenge Method) or by introduction by a tick vector (Tick Challenge Method).

The Needle Challenge Method is carried out for the desired Borrelia strain (e.g., B. burgdorferi, strain ZS7) by subcutaneously introducing Borrelia at a dose between 20 and 50 times the Infectious Dose (ID)₅₀ to mice that are immunized with said first polypeptide of the first aspect or with an appropriate placebo (negative) control, such as buffer or adjuvant alone and comparing the rates of infection in the challenged mice. The ID₅₀ is defined as the dose at which 50% of the challenged mice are infected. The dose of Borrelia is measured in numbers of bacteria. The challenge dose can vary widely and is strain-dependent; therefore, the virulence of the strain must first be assessed by challenge experiments for determination of ID₅₀. Four weeks after needle challenge, blood and tissues are collected for readout methods to determine the infection status. These readout methods can be e.g. VlsE ELISA on sera or qPCR on collected tissues for identification of Borrelia, as described herein, or other methods.

The Tick Challenge Method is carried out by applying at least one tick nymph (e.g., I. ricinus) infected with Borrelia (e.g., B. afzelii, strain IS1), to a mouse that is immunized with said first polypeptide of the first aspect; and b) applying at least one infected tick nymph to a second mouse that is immunized with said second polypeptide of the first aspect; and c) comparing the rates of infection in the two mice, generally six weeks after challenge. Preferably, the assay or test is done with a group of mice per polypeptide to be tested. A suitable test is also described and illustrated in the Examples. Assessment of infection status can be done using VlsE ELISA on sera or qPCR on DNA isolated from collected tissues, or using other suitable methods.

In a preferred embodiment of the present invention, the products of the invention such as, e.g. the polypeptides of the invention comprising the hybrid OspA fragment and preferably the mutant OspA C-terminal fragment administered 3 times to a subject at a dose of 30 μg, preferably 10 μg, preferably 5.0 μg, preferably 1.0 μg, preferably 0.3 μg or lower have a protective capacity of 50% or more, preferably 60% or more, more preferably 70% or more, more preferably 80% or more, more preferably 90% or more, even more preferably 95% or more, most preferred 99% or more. In one embodiment, the protective capacity is assessed in an in vivo challenge method, preferably a Tick Challenge Method, more preferably a Needle Challenge Method, e.g. as described in the Examples.

In a preferred embodiment, the difference in protective capacity (Δpc) between the polypeptides of the invention comprising the hybrid OspA C-terminal fragment and the placebo (negative) control is at least 50%, especially at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, even more preferably at least 95%, most preferably at least 99%, when administered 3 times to a subject at a dose of 30 μg, preferably 10 μg, preferably 5.0 μg, preferably 1.0 μg, preferably 0.3 μg or lower.

In accordance with the present invention, the first part of the hybrid OspA may be from any Borrelia strain other than B. garinii, strain PBr, with SEQ ID NO: 8, particularly from those specified herein such as B. burgdorferi s.s., B. garinii (not strain PBr), B. afzelii, B. andersoni, B. bissettii, B. valaisiana, B. lusitaniae, B. spielmanii, B. japonica, B. tanukii, B. turdi or B. sinica, B. bavariensis, preferably from B. burgdorferi s.s., B. afzelii, B. bavariensis or B. garinii, or a fusion of OspA protein fragments from two or more of these species. Preferably, the OspA is from B. valaisiana, particularly strain VS116 (SEQ ID NO: 4) but may be from B. afzelii, particularly strain K78, OspA serotype 2 (SEQ ID NO: 6); B. burgdorferi s.s., particularly strain B31, OspA serotype 1 (SEQ ID NO: 5); B. garinii, particularly strain PBr, OspA serotype 3 (SEQ ID NO: 8); B. bavariensis, particularly strain PBi, OspA serotype 4 (SEQ ID NO: 9); B. garinii, particularly strain PHei, OspA serotype 5 (SEQ ID NO: 10); B. garinii, particularly strain DK29, OspA serotype 6 (SEQ ID NO: 11) or B. garinii, particularly strain T25, OspA serotype 7 (SEQ ID NO: 12). The amino acid sequences of these OspA proteins (full-length) are given below.

In accordance with the present invention, the disulfide bond may also be formed between cysteines that have been introduced at any position of the OspA fragment allowing or supporting appropriate folding of the fragment. The positions may be selected, as detailed above, based on the known structure of the OspA. In a preferred embodiment, the polypeptide of the current invention contains at least one disulfide bond introduced by the insertion of a cysteine residue at one of residues 182+/−3 and one of residues 269+/−3 (disulfide bond type 1; “D1”) of a B. afzelii, particularly B. afzelii K78 serotype 2 OspA, or the homologous amino acids of an OspA from a Borrelia other than B. afzelii, such as B. burgdorferi s.s., particularly strain B31, serotype 1; B. garinii, particularly strain PBr, serotype 3; B. bavariensis, particularly strain PBi, serotype 4; B. garinii, particularly strain PHei, serotype 5; B. garinii, particularly strain DK29, serotype 6; B. garinii, particularly strain T25, serotype 7 or a fusion of amino acids 125-176 of OspA of B. valaisiana, strain VS116, or amino acids 126-175 of OspA of B. spielmanii and amino acids 177-274 of B. garinii, strain PBr (SEQ ID NO: 8).

It is noted that:

Position 182+/−3 is an abbreviation for position 179, 180, 181, 182, 183, 184 or 185, preferably 182. Position 269+/−3 is an abbreviation for position 266, 267, 268, 269, 270, 271 or 272, preferably 269.

In a preferred embodiment, the additional mutant fragment is derived from the amino acids from position 125, 126, 130 or 131 to position 273 of the wild-type sequence of the OspA of B. afzelii strain K78, serotype 2 (SEQ ID NO: 6) and differs only by the introduction of at least one disulfide bond, particularly wherein the at least one disulfide bond is between positions 182 and 269 (disulfide bond type 1); or the homologous fragments and positions of an OspA from a Borrelia sp. other than B. afzelii, such as B. burgdorferi s.s., particularly strain B31, serotype 1; B. garinii, particularly strain PBr, serotype 3; B. bavariensis, particularly strain PBi, serotype 4; B. garinii, particularly strain PHei, serotype 5; B. garinii, particularly strain DK29, serotype 6 or B. garinii, particularly strain T25, serotype 7.

In a further embodiment, the mutant fragment may be an amino acid sequence selected from the group consisting of SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, most preferably SEQ ID NO: 46, and an amino acid sequence that has at least 80%, more preferably at least 85%, more preferably at least 90%, even more preferably at least 95% sequence identity to at least one of sequences with SEQ ID NOs: 19 to 25, wherein the cysteines are not replaced. Further details on mutations and sequence identity are given above.

As detailed above, the polypeptide of the present invention may comprise signal sequences. It has been shown that lipidation confers adjuvant properties on OspA. Accordingly, lipidated forms of the polypeptide of the invention or polypeptides comprising a lipidation signal are preferred. In a preferred embodiment, the polypeptide of the current invention comprises a lipidation signal, preferably a lipidation signal of a Borrelia outer surface protein, OspA or OspB (SEQ ID NOs: 13 and 14, respectively) or more preferably an E. coli 1pp lipidation signal sequence (SEQ ID NO: 15). The OspA fragment of the invention comprising a lipidation signal is lipidated during processing and the lipidation signal peptide is cleaved off; therefore, the signal peptide is no longer present in the mature lipidated protein.

Lipidated proteins according to the current invention are labeled with “Lip” at the N-terminus to indicate the addition of 3 fatty acid groups and a glycerol to the polypeptide. Suitable lipidation signals as described above include MKKYLLGIGLILALIA (SEQ ID NO: 13), MRLLIGFALALALIG (SEQ ID NO: 14) and MKATKLVLGAVILGSTLLAG (SEQ ID NO: 15). Because lipid moieties and a glycerol are attached to the N-terminal cysteine residue which is present in the full-length wild-type OspA protein, OspA C-terminal fragments for lipidation may additionally comprise a peptide comprising a cysteine residue followed by additional amino acids. For example, sequences such as CSS or CKQN (SEQ ID NO: 62) immediately C-terminal to the lipidation signal sequence provide an N-terminal cysteine residue for lipidation upon cleavage of the lipidation signal peptide. The lipidated cysteine-containing peptides are present in the final lipidated polypeptide of the invention.

It has been speculated that the OspA protein of B. burgdorferi s.s. comprises a sequence with the capacity to bind to a T cell receptor that also has the capacity to bind to human leukocyte function-associated antigen (hLFA-1) (herein referred to also as “hLFA-1-like sequence”). The similarity of this OspA region to hLFA-1 may result in an immune response with cross-reactivity upon administration of B. burgdorferi s.s. OspA to a human subject and may induce autoimmune diseases, particularly autoimmune arthritis, in susceptible individuals. Accordingly, in a preferred embodiment, the polypeptide of the current invention does not comprise a sequence with binding capacity to the T cell receptor that has a binding capacity to the human leukocyte function-associated antigen (hLFA-1), and particularly does not comprise the amino acid sequence GYVLEGTLTAE (SEQ ID NO: 17). To this end, the hLFA-1-like sequence, particularly the amino acid sequence GYVLEGTLTAE (SEQ ID NO: 17), may be replaced with a homologous sequence from an OspA protein of another Borrelia sp., particularly with NFTLEGKVAND (SEQ ID NO: 18).

In a preferred embodiment, the polypeptide of the current invention comprising at least one disulfide bond essentially establishes the same protective capacity with said polypeptide against a Borrelia infection relative to at least one of the wild-type full-length OspA proteins derived from at least one 20 Borrelia strain, particularly B. afzelii K78, OspA serotype 2 (SEQ ID NO: 6); B. burgdorferi s.s., particularly strain B31, serotype 1 (SEQ ID NO: 5); B. garinii, particularly strain PBr, serotype 3 (SEQ ID NOs: 7 and 8); B. bavariensis, particularly strain PBi, serotype 4 (SEQ ID NO: 9); B. garinii, particularly strain PHei, serotype 5 (SEQ ID NO: 10); B. garinii, particularly strain DK29, serotype 6 (SEQ ID NO: 11) or B. garinii, particularly strain T25, serotype 7 (SEQ ID NO: 12).

Please note that further details on mutations and sequence identity are given above.

TABLE A-3 Nomenclature and SEQ ID NOs. of the lipidated mutant OspA fragment heterodimers described in the current invention. Lipidated mutant OspA fragment heterodimer* SEQ ID NO: Lip-S1D1-S2D1 29 Lip-S5D1-S6D1 33 Lip-S4D1-S3D1 31 Lip-S4D1-S3hybD1 27 *S = Serotype (1-6) (see Table A-2); S3hyb = fusion of amino acids 125-176 of B. valaisiana and amino acids 177-274 of B. garinii, strain PBr D1 = Disulfide Bond Type 1 (cysteine residues inserted at position 183 +/− 3 and 270 +/− 3); Lip = lipidation: the N-terminal addition of glycerol and fatty acid residues.

In another preferred embodiment, the polypeptide according to the first aspect comprises at least two or three mutant fragments which are connected via one or more linkers. A linker is a rather short amino acid sequence employed to connect two fragments. It should be designed in order to avoid any negative impact on the fragments, their interaction in subjects to be treated or vaccinated or upon their protective capacity. Preferred are short linkers of at most 21 amino acids, particularly at most 15 amino acids, especially at most 12 or 8 amino acids. More preferably, the one or more linkers is/are composed of small amino acids in order to reduce or minimize interactions with the fragments, such as glycine, serine and alanine. A preferred linker is the “LN1” peptide linker, a fusion of two separate loop regions of the N-terminal half of OspA from B. burgdorferi s.s., strain B31 (aa 65-74 and aa 42-53, with an amino acid exchange at position 53 of D53S) which has the following sequence: GTSDKNNGSGSKEKNKDGKYS (SEQ ID NO: 16). The linker may comprise a lipitation site.

In another preferred embodiment, the polypeptide according to the first aspect comprises a polypeptide with a total size of at most 500 amino acids, comprising two or three different mutant fragments as defined in preferred embodiments of the first aspect; or a polypeptide which consists of essentially two or three different mutant fragments, one or two linkers and, optionally, an N-terminal cysteine; and/or a polypeptide which consists of essentially two or three different mutant fragments, an N-terminal extension of the fragment consisting of at most 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12 or 11 amino acids, preferably at most 10, 9, 8, 7 or 6 amino acids, still more preferably at most 5, 4, 3, 2 or 1 amino acid(s), wherein the N-terminal extension is located directly N-terminally from the fragment in the respective Borrelia OspA and, optionally, an N-terminal cysteine. The N-terminal cysteine may optionally be followed by a short peptide linker from 1-10 amino acids long, and preferably takes the form of an N-terminal CSS peptide.

The Nucleic Acids of the Invention and Related Aspects

In a further aspect, the present invention relates to a nucleic acid coding for a polypeptide as defined herein and above in the context of the present invention. The nucleic acid may be comprised in a vector and/or cell.

The invention further provides a nucleic acid encoding a polypeptide of the invention. For the purposes of the invention the term “nucleic acid(s)” generally refers to any polyribonucleotide or polydeoxyribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA including single and double-stranded regions/forms.

The term “nucleic acid encoding a polypeptide” as used herein encompasses polynucleotides that include a sequence encoding a peptide or polypeptide of the invention. The term also encompasses polynucleotides that include a single continuous region or discontinuous regions encoding the peptide or polypeptide (for example, polynucleotides interrupted by integrated phage, an integrated insertion sequence, an integrated vector sequence, an integrated transposon sequence, or due to RNA editing or genomic DNA reorganization) together with additional regions, that also may contain coding and/or non-coding sequences.

It will be appreciated by those of ordinary skill in the art that, as a result of the degeneracy of the genetic code, there are many nucleotide sequences that encode a polypeptide as described herein.

Some of these polynucleotides bear minimal similarity to the nucleotide sequence of any native (i.e., naturally occurring) gene. Nonetheless, polynucleotides that vary due to differences in codon usage are specifically contemplated by the present invention, for example polynucleotides that are optimized for human and/or primate and/or E. coli codon selection.

Sequences encoding a desired polypeptide may be synthesized, in whole or in part, using chemical methods well known in the art (see Caruthers, M. H. et al., Nucl. Acids Res. Symp. Ser. pp. 215-223 (1980), Horn et al., Nucl. Acids Res. Symp. Ser. pp. 225-232 (1980)). Alternatively, the protein itself may be produced using chemical methods to synthesize the amino acid sequence of a polypeptide, or a portion thereof. For example, peptide synthesis can be performed using various solid-phase techniques (Roberge et al., Science 269:202-204 (1995)) and automated synthesis may be achieved, for example, using the ASI 431 A Peptide Synthesizer (Perkin Elmer, Palo Alto, CA).

Moreover, the polynucleotide sequences of the present invention can be engineered using methods generally known in the art in order to alter polypeptide encoding sequences for a variety of reasons, including but not limited to, alterations which modify the cloning, processing, and/or expression of the gene product. For example, DNA shuffling by random fragmentation and PCR reassembly of gene fragments and synthetic oligonucleotides may be used to engineer the nucleotide sequences. In addition, site-directed mutagenesis may be used to insert new restriction sites, alter glycosylation patterns, change codon preference, produce splice variants, or introduce mutations, and so forth.

In a further aspect of the invention the present invention relates to vectors comprising a nucleic acid of the invention, e.g. linked to an inducible promoter such that when the promoter is induced a polypeptide encoded by the nucleic acid is expressed. In a preferred embodiment, the vector is pET28b(+) (http://www.addgene.org/vector-database/2566/).

A further aspect of the invention comprises said vector wherein the inducible promoter is activated by addition of a sufficient quantity of IPTG (Isopropyl β-D-1-thiogalactopyranoside) preferably to the growth medium. Optionally this is at a concentration of between 0.1 and 10 mM, 0.1 and 5 mM, 0.1 and 2.5 mM, 0.2 and 10 mM, 0.2 and 5 mM, 0.2 and 2.5 mM, 0.4 and 10 mM, 1 and 10 mM, 1 and 5 mM, 2.5 and 10 mM, 2.5 and 5 mM, 5 and 10 mM. Alternatively the promoter may be induced by a change in temperature or pH.

Nucleic acid molecule as used herein generally refers to any ribonucleic acid molecule or deoxyribonucleic acid molecule, which may be unmodified RNA or DNA or modified RNA or DNA. Thus, for instance, nucleic acid molecule as used herein refers to at least single- and double-stranded DNA, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded, or a mixture of single- and double-stranded regions. As used herein, the term nucleic acid molecule includes DNA or RNA molecules as described above that contain one or more modified bases. Thus, DNA or RNA molecules with backbones modified for stability or for other reasons are “nucleic acid molecule” as that term is intended herein. Moreover, DNA or RNA species comprising unusual bases, such as inosine, or modified bases, such as tritylated bases, to name just two examples, are also nucleic acid molecules as defined herein. It will be appreciated that a great variety of modifications have been made to DNA and RNA molecules that serve many useful purposes known to those of skill in the art. The term nucleic acid molecule as used herein embraces such chemically, enzymatically or metabolically modified forms of nucleic acid molecules, as well as the chemical forms of DNA and RNA characteristic of viruses and cells, including simple and complex cells, inter alia. The term nucleic acid molecule also encompasses short nucleic acid molecules often referred to as oligonucleotide(s). The terms “polynucleotide” and “nucleic acid” or “nucleic acid molecule” are used interchangeably herein.

The nucleic acids according to the present invention may be chemically synthesized. Alternatively, the nucleic acids can be isolated from Borrelia and modified by methods known to one skilled in the art. The same applies to the polypeptides according to the present invention.

Furthermore, the nucleic acid of the present invention can be functionally linked, using standard techniques such as cloning, to any desired sequence(s), whether a Borrelia regulatory sequence or a heterologous regulatory sequence, heterologous leader sequence, heterologous marker sequence or a heterologous coding sequence to create a fusion gene.

Nucleic acid molecules of the present invention may be in the form of RNA, such as mRNA or cRNA, or in the form of DNA, including, for instance, cDNA and genomic DNA obtained by cloning or produced by chemical synthesis techniques or by a combination thereof. The DNA may be triple-stranded, double-stranded or single-stranded. Single-stranded DNA may be the coding strand, also known as the sense strand, or it may be the non-coding strand, also referred to as the anti-sense strand.

The nucleic acid of the present invention may be comprised in a vector or in a host cell. Accordingly, the present invention also relates to a vector or a host cell, preferably E. coli, comprising a nucleic acid molecule according to the present invention. The vector may comprise the above-mentioned nucleic acid in such a manner that the vector is replicable and can express the protein encoded by the nucleotide sequence in a host cell.

For recombinant production of the polypeptides of the invention, host cells can be genetically engineered to incorporate expression systems or portions thereof of the nucleic acid of the invention. Introduction of a nucleic acid into a host cell can be effected by methods described in many standard laboratory manuals, such as Davis, et al., BASIC METHODS IN MOLECULAR BIOLOGY, (1986) and Sambrook, et al., MOLECULAR CLONING: A LABORATORY MANUAL, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989), such as, calcium phosphate transfection, DEAE-dextran mediated transfection, transvection, microinjection, cationic lipid-mediated transfection, electroporation, conjugation, transduction, scrape loading, ballistic introduction and infection.

Representative examples of appropriate hosts include gram negative bacterial cells, such as cells of E. coli, Acinetobacter, Actinobacillus, Bordetella, Brucella, Campylobacter, Cyanobacteria, Enterobacter, Erwinia, Franciscella, Helicobacter, Hemophilus, Klebsiella, Legionella, Moraxella, Neisseria, Pasteurella, Proteus, Pseudomonas, Salmonella, Serratia, Shigella, Treponema, Vibrio, Yersinia. In one embodiment, the host cell is an Escherichia coli cell. In a preferred embodiment, the host cell is an E. coli BL21(DE3) cell or an E. coli BL21 Star™ (DE3) cell.

Alternatively, gram positive bacterial cells may also be used. A great variety of expression systems can be used to produce the polypeptides of the invention. In one embodiment the vector is derived from bacterial plasmids. Generally any system or vector suitable to maintain, propagate or express polynucleotides and/or to express a polypeptide in a host may be used for expression in this regard. The appropriate DNA sequence may be inserted into the expression system by any of a variety of well-known and routine techniques, such as, for example, those set forth in Sambrook et al., MOLECULAR CLONING, A LABORATORY MANUAL (supra).

In one embodiment of the current invention, the cells are grown under selective pressure, such as in the presence of antibiotics, preferably kanamycin. In another embodiment, cells are grown in the absence of antibiotics.

A great variety of expression vectors can be used to express the polypeptides according to the present invention. Generally, any vector suitable to maintain, propagate or express nucleic acids to express a polypeptide in a host may be used for expression in this regard. In accordance with this aspect of the invention the vector may be, for example, a plasmid vector, a single- or double-stranded phage vector or a single- or double-stranded RNA or DNA viral vector. Starting plasmids disclosed herein are either commercially available, publicly available, or can be constructed from available plasmids by routine application of well-known, published procedures. Preferred among vectors, in certain respects, are those for expression of nucleic acid molecules and the polypeptides according to the present invention. Nucleic acid constructs in host cells can be used in a conventional manner to produce the gene product encoded by the recombinant sequence. Alternatively, the polypeptides according to the present invention can be synthetically produced by conventional peptide synthesizers.

In addition, the present invention relates to a host cell comprising this vector. Representative examples of appropriate host cells include bacteria, such as streptococci, staphylococci, E. coli, Streptomyces and Bacillus subtilis; fungi, such as yeast and Aspergillus; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; mammalian cells such as CHO, COS, HeLa, C127, 3T3, BHK, 293 or Bowes melanoma cells; and plant cells. Cell-free translation systems can also be employed to produce such proteins using RNA derived from the DNA construct of the present invention.

In order to express the desired amino acid sequence practically by introducing the vector according to the present invention into a host cell, the vector may contain, in addition to the nucleic acid sequence according to the present invention, other sequences for controlling the expression (e.g., promoter sequences, terminator sequences and enhancer sequences) and gene markers for selecting microorganisms, insect cells, animal culture cells, or the like (e.g., neomycin resistance genes and kanamycin resistance genes). Furthermore, the vector may contain the nucleic acid sequence according to the present invention in a repeated form (e.g., in tandem). The vector may be constructed based on procedures which are conventionally used in the field of genetic engineering.

The host cells may be cultured in an appropriate medium, and the protein according to the present invention may be obtained from the culture product. The protein according to the present invention may be recovered from the culture medium and purified in the conventional manner

Accordingly, the present invention also relates to a process for producing a cell which expresses a polypeptide according to the present invention, comprising transforming or transfecting a suitable host cell with the vector according to the present invention or a process for producing the polypeptide according to the present invention, comprising expressing the nucleic acid molecule according to the present invention.

Alternatively, a method for producing a polypeptide as defined above may be characterized by the following steps:

-   -   a) introducing a vector encoding the polypeptide into a host         cell,     -   b) growing the host cell under conditions allowing for         expression of said polypeptide,     -   c) homogenizing said host cell, and     -   d) subjecting the host cell homogenate to purification steps.

The invention further relates to a method for producing a polypeptide as defined above, characterized by the following steps:

-   -   a) introducing a nucleic acid encoding a polypeptide into a         vector,     -   b) introducing said vector into a host cell,     -   c) growing said host cell under conditions allowing for         expression of polypeptide,     -   d) homogenizing said host cell,     -   e) enriching polypeptide in the lipid phase by phase separation,         and     -   f) further purifying over a gel filtration column.

The invention further relates to a method for producing a polypeptide as defined above, characterized by the following steps:

-   -   a) introducing a nucleic acid encoding a polypeptide into a         vector,     -   b) introducing said vector into a host cell,     -   c) growing said host cell under conditions allowing for         expression of polypeptide,     -   d) homogenizing said host cell,     -   e) enriching polypeptide in the lipid phase by phase separation,     -   g) purifying over a gel filtration column, and     -   h) optionally, further processing over a buffer exchange column.

The Antibodies of the Invention and Related Aspects

The problem underlying the present invention is solved in a further aspect by an antibody, or at least an effective part thereof, which selectively binds to the hybrid C-terminal OspA fragment as defined in the context of the present invention, but not to the first OspA portion and the second OspA portion alone (i.e. if not fused to the other portion).

In a preferred embodiment the antibody is a monoclonal antibody.

In another preferred embodiment said effective part comprises an Fab fragment, an F(ab) fragment, an F(ab)N fragment, an F(ab)₂ fragment or an F_(v) fragment or single domain antobody.

In still another embodiment of the invention the antibody is a chimeric antibody.

In yet another embodiment the antibody is a humanized antibody.

In a preferred aspect, antibodies of the invention bind specifically to hybrid OspA fragment polypeptides of the invention, but not to the first OspA portion and the second OspA portion alone and not to corresponding wild-type OspA fragment polypeptides. In a more preferred aspect, the antibody binds specifically to the disulfide bond of the mutant OspA fragment of the invention.

The term “specificity” refers to the number of different types of antigens or antigenic determinants to which a particular antigen-binding molecule or antigen-binding protein (such as a Nanobody or a polypeptide of the invention) can bind. The specificity of an antigen-binding protein can be determined based on affinity and/or avidity. The affinity, represented by the equilibrium constant for the dissociation of an antigen with an antigen-binding protein (K_(D)), is a measure for the binding strength between an antigenic determinant and an antigen-binding site on the antigen-binding protein: the lesser the value of the K_(D), the stronger the binding strength between an antigenic determinant and the antigen-binding molecule (alternatively, the affinity can also be expressed as the affinity constant (K_(A)), which is 1/K_(D)).

As will be clear to the skilled person (for example on the basis of the further disclosure herein), affinity can be determined in a manner known per se, depending on the specific antigen of interest. Avidity is the measure of the strength of binding between an antigen-binding molecule (such as an antibody or an effective part thereof of the invention) and the pertinent antigen. Avidity is related to both the affinity between an antigenic determinant and its antigen binding site on the antigen-binding molecule and the number of pertinent binding sites present on the antigen-binding molecule. Typically, antigen-binding proteins (such as an antibody or an effective part thereof of the invention) will bind to their antigen with a dissociation constant (K_(D)) of 10⁻⁵ to 10⁻¹² M or less, and preferably 10⁻⁷ to 10⁻¹² M or less and more preferably 10⁻⁸ to 10⁻¹² M (i.e. with an association constant (K_(A)) of 10⁵ to 10¹² M⁻¹ or more, and preferably 10⁸ to 10¹² liter/moles or more and more preferably 10⁸ to 10¹² M⁻¹). Any K_(D) value greater than 10⁴M (or any K_(A) value lower than 10⁴M⁻¹) is generally considered to indicate non-specific binding. Preferably, a monovalent immunoglobulin sequence of the invention will bind to the desired antigen with an affinity less than 500 nM, preferably less than 200 nM, more preferably less than 10 nM, such as less than 500 pM. Specific binding of an antigen-binding protein to an antigen or antigenic determinant can be determined in any suitable manner known per se, including, for example, Scatchard analysis and/or competitive binding assays, such as radioimmunoassays (RIA), enzyme immunoassays (EIA) and sandwich competition assays, and the different variants thereof known per se in the art, as well as the other techniques mentioned herein.

The dissociation constant may be the actual or apparent dissociation constant, as will be clear to the skilled person. Methods for determining the dissociation constant will be clear to the skilled person, and for example include the techniques mentioned herein. In this respect, it will also be clear that it may not be possible to measure dissociation constants of more than 10⁻⁴M or 10⁻³M (e.g., of 10⁻² M). Optionally, as will also be clear to the skilled person, the (actual or apparent) dissociation constant may be calculated on the basis of the (actual or apparent) association constant (K_(A)), by means of the relationship [K_(D)=1/K_(A)].

The affinity denotes the strength or stability of a molecular interaction. The affinity is commonly given as by the K_(D), or dissociation constant, which has units of mole/liter (or M). The affinity can also be expressed as an association constant, K_(A), which equals 1/K_(D) and has units of (liter/mole)⁻¹ (or M⁻¹). In the present specification, the stability of the interaction between two molecules (such as an amino acid sequence, Nanobody or polypeptide of the invention and its intended target) will mainly be expressed in terms of the K_(D) value of their interaction; it being clear to the skilled person that in view of the relation K_(A)=1/K_(D), specifying the strength of molecular interaction by its K_(D) value can also be used to calculate the corresponding K_(A) value. The K_(D) value characterizes the strength of a molecular interaction also in a thermodynamic sense as it is related to the free energy (DG) of binding by the well known relation DG=RT·ln(K_(D)) (equivalently DG=−RT·ln(K_(A))), where R equals the gas constant, T equals the absolute temperature and In denotes the natural logarithm.

The K_(D) for biological interactions which are considered meaningful (e.g. specific) are typically in the range of 10⁻¹⁰ M (0.1 nM) to 10⁻⁵ M (10000 nM). The stronger an interaction, the lower its K_(D).

In a preferred embodiment, the K_(D) of the antibody of the invention is between 10⁻¹² M and 10⁻⁵ M, preferably less than 10⁻⁶, preferably less than 10⁻⁷, preferably less than 10⁻⁸ M, preferably less than 10⁻⁹ M, more preferably less than 10⁻¹⁰ M, even more preferably less than 10⁻¹¹ M, most preferably less than 10⁻¹²M.

The K_(D) can also be expressed as the ratio of the dissociation rate constant of a complex, denoted as k_(off), to the rate of its association, denoted k. (so that K_(D)=k_(off)/k_(on) and K_(A)=k_(on)/k_(off)). The off-rate k_(off) has units s⁻¹ (where s is the SI unit notation for second). The on-rate k_(on) has units M⁻¹ s⁻¹. The on-rate may vary between 10² M⁻¹ s⁻¹ to about 10⁷ M⁻¹s⁻¹, approaching the diffusion-limited association rate constant for bimolecular interactions. The off-rate is related to the half-life of a given molecular interaction by the relation t_(1/2)=ln(2)/k_(off). The off-rate may vary between 10⁻⁶ s⁻¹ (near irreversible complex with a t_(1/2) of multiple days) to 1 s⁻¹(t_(1/2)=0.69 s).

The affinity of a molecular interaction between two molecules can be measured via different techniques known per se, such as the well known surface plasmon resonance (SPR) biosensor technique (see for example Ober et al., Intern Immunology, 13, 1551-1559, 2001) where one molecule is immobilized on the biosensor chip and the other molecule is passed over the immobilized molecule under flow conditions yielding k_(on), k_(off) measurements and hence K_(D) (or K_(A)) values. This can for example be performed using the well-known BIACORE instruments.

It will also be clear to the skilled person that the measured K_(D) may correspond to the apparent K_(D) if the measuring process somehow influences the intrinsic binding affinity of the implied molecules for example by artefacts related to the coating on the biosensor of one molecule. Also, an apparent K_(D) may be measured if one molecule contains more than one recognition site for the other molecule. In such situation the measured affinity may be affected by the avidity of the interaction by the two molecules.

Another approach that may be used to assess affinity is the 2-step ELISA (Enzyme-Linked Immunosorbent Assay) procedure of Friguet et al. (J. Immunol. Methods, 77, 305-19, 1985). This method establishes a solution phase binding equilibrium measurement and avoids possible artefacts relating to adsorption of one of the molecules on a support such as plastic.

However, the accurate measurement of K_(D) may be quite labor-intensive; therefore, apparent K_(D) values are often determined in order to assess the binding strength of two molecules. It should be noted that as long as all measurements are made in a consistent way (e.g. keeping the assay conditions unchanged), apparent K_(D) measurements can be used as an approximation of the true K_(D) and hence in the present document K_(D) and apparent K_(D) should be treated with equal importance or relevance.

Finally, it should be noted that in many situations the experienced scientist may judge it to be convenient to determine the binding affinity relative to some reference molecule. For example, to assess the binding strength between molecules A and B, one may e.g. use a reference molecule C that is known to bind to B and that is suitably labelled with a fluorophore or chromophore group or other chemical moiety, such as biotin for easy detection in ELISA or flow cytometry or other format (the fluorophore for fluorescence detection, the chromophore for light absorption detection, the biotin for streptavidin-mediated ELISA detection). Typically, the reference molecule C is kept at a fixed concentration and the concentration of A is varied for a given concentration or amount of B. As a result an Inhibitory Concentration (IC)₅₀ value is obtained corresponding to the concentration of A at which the signal measured for C in absence of A is halved. Provided K_(D ref), the K_(D) of the reference molecule, is known, as well as the total concentration c_(ref) of the reference molecule, the apparent K_(D) for the interaction A-B can be obtained from following formula: K_(D)=IC₅₀/(1 c_(ref)/K_(Dref)). Note that if c_(ref)<<K_(Dref), K_(D)≈IC₅₀. Provided the measurement of the IC₅₀ is performed in a consistent way (e.g. keeping c_(ref) fixed) for the binders that are compared, the strength or stability of a molecular interaction can be assessed by the IC₅₀ and this measurement is judged as equivalent to K_(D) or to apparent K_(D) throughout this text.

Another aspect of the invention relates to a hybridoma cell line, which produces an antibody as defined above.

The problem underlying the present invention is furthermore solved by a method for producing an antibody as defined above, characterized by the following steps:

-   -   a) initiating an immune response in a non-human animal by         administering a polypeptide as defined above to said animal,     -   b) removing an antibody containing body fluid from said animal,         and     -   c) producing the antibody by subjecting said antibody containing         body fluid to further purification steps.

The invention further relates to a method for producing an antibody as defined above, characterized by the following steps:

-   -   a) initiating an immune response in a non-human animal by         administering a polypeptide as defined above to said animal,     -   b) removing the spleen or spleen cells from said animal,     -   c) producing hybridoma cells of said spleen or spleen cells,     -   d) selecting and cloning hybridoma cells specific for said         polypeptide,     -   e) producing the antibody by cultivation of said cloned         hybridoma cells, and     -   f) optionally conducting further purification steps.

Pharmaceutical Compositions and Related Medical Aspects

Another aspect of the present invention is related to a pharmaceutical composition comprising

-   -   (i) the polypeptide according to the present invention, the         nucleic acid according to the present invention, and/or the         antibody according to the present invention; and     -   (ii) optionally a pharmaceutically acceptable excipient.

Accordingly, the polypeptide according to the present invention, the nucleic acid according to the present invention, the antibody according to the present invention or the pharmaceutical composition according to the present invention may be

-   -   for use as a medicament, particularly as a vaccine or     -   for use in a method of treating or preventing a Borrelia         infection, particularly a B. burgdorferi s.s., B. garinii, B.         afzelii, B. andersoni, B. bavariensis, B. bissettii, B.         valaisiana, B. lusitaniae, B. spielmanii, B. japonica, B.         tanukii, B. turdi or B. sinica infection, preferably a B.         burgdorferi s.s., B. afzelii or B. garinii infection.

Preferably, the composition additionally comprises Lip-S1D1-S2D1 (SEQ ID NO: 29) and Lip-S5D1-S6D1 (SEQ ID NO: 33), the composition additionally comprises Lip-S1D1-S2D1 (SEQ ID NO: 29), the composition additionally comprises Lip-S5D1-S6D1 (SEQ ID NO: 33), or the composition comprises Lip-S1D1-S2D1 (SEQ ID NO: 29), Lip-S4D1-S3hybD1 (SEQ ID NO: 27) and Lip-S5D1-S6D1 (SEQ ID NO: 33).

Still another aspect relates to a pharmaceutical composition as defined above for use in the treatment or prevention of an infection with Borrelia species, more preferably pathogenic Borrelia species as disclosed herein more preferably comprising B. burgdorferi s.s., B. afzelii, B. bavariensis and B. garinii.

The problem underlying the present invention is solved in another aspect by the use of the polypeptide according to the present invention, the nucleic acid according to the present invention, and/or the antibody according to the present invention for the preparation of a pharmaceutical composition for treating or preventing infections with Borrelia species, more preferably pathogenic Borrelia species as disclosed herein more preferably comprising B. burgdorferi s.s., B. afzelii, B. bavariensis and B. garinii.

The pharmaceutical composition may optionally contain any pharmaceutically acceptable carrier or excipient, such as buffer substances, stabilisers or further active ingredients, especially ingredients known in connection with pharmaceutical compositions and/or vaccine production. Preferably, the pharmaceutically acceptable excipient comprises L-methionine. Preferably, the pharmaceutical composition is for use as a medicament, particularly as a vaccine or for preventing or treating an infection caused by Borrelia species, more preferably pathogenic Borrelia species as disclosed herein more preferably comprising B. burgdorferi s.s., B. afzelii, B. bavariensis and B. garinii, and/or other pathogens against which the antigens have been included in the vaccine. Preferably, the pharmaceutical composition is for use in a method of treating or preventing a Borrelia infection, particularly a B. burgdorferi s.s., B. garinii, B. afzelii, B. andersoni, B. bavariensis, B. bissettii, B. valaisiana, B. lusitaniae, B. spielmanii, B. japonica, B. tanukii, B. turdi or B. sinica infection, preferably a B. burgdorferi s.s., B. afzelii or B. garinii infection.

In one embodiment the pharmaceutical composition further comprises an adjuvant. The choice of a suitable adjuvant to be mixed with bacterial toxins or conjugates made using the processes of the invention is within the knowledge of the person skilled in the art. Suitable adjuvants include an aluminium salt such as aluminium hydroxide or aluminum phosphate, but may also be other metal salts such as those of calcium, magnesium, iron or zinc, or may be an insoluble suspension of acylated tyrosine, or acylated sugars, cationically or anionically derivatized saccharides, or polyphosphazenes. In a preferred embodiment, the pharmaceutical composition is adjuvanted with aluminium hydroxide.

In a further embodiment, the pharmaceutical composition further comprises an immunostimulatory substance, preferably selected from the group consisting of polycationic polymers, especially polycationic peptides, immunostimulatory oligodeoxynucleotides (ODNs), especially oligo(dIdC)₁₃ (SEQ ID NO: 63), peptides containing at least two LysLeuLys motifs, especially peptide KLKLLLLLKLK (SEQ ID NO: 61), neuroactive compounds, especially human growth hormone, aluminium hydroxide, aluminium phosphate, Freund's complete or incomplete adjuvants, or combinations thereof. Preferably, the immunostimulatory substance is a combination of either a polycationic polymer and immunostimulatory deoxynucleotides or of a peptide containing at least two LysLeuLys motifs and immunostimulatory deoxynucleotides, preferably a combination of KLKLLLLLKLK (SEQ ID NO: 61) and oligo(dIdC)₁₃ (SEQ ID NO: 63). More preferably, said polycationic peptide is polyarginine.

In a further embodiment, the pharmaceutical composition comprises sodium phosphate, sodium chloride, L-methionine, sucrose and Polysorbate-20 (Tween-20) at a pH of 6.7+/−0.2. Preferably, the pharmaceutical composition also comprises aluminium hydroxide, preferably at a concentration of 0.15%.

In one embodiment, the formulation comprises between 5 mM and 50 mM sodium phosphate, between 100 and 200 mM sodium chloride, between 5 mM and 25 mM L-Methionine, between 2.5% and 10% Sucrose, between 0.01% and 0.1% Tween 20 and between 0.1% and 0.2% (w/v) aluminium hydroxide. More preferably, the formulation comprises 10 mM sodium phosphate, 150 mM sodium chloride, 10 mM L-Methionine, 5% Sucrose, 0.05% Tween 20 and 0.15% (w/v) aluminium hydroxide at pH 6.7±0.2. Even more preferably, the formulation comprises at least one, at least two, at least three mutant OspA heterodimers according to the invention.

In one embodiment, the pharmaceutical composition comprises 3 heterodimers, preferably Lip-S1D1-S2D1 (SEQ ID NO: 29), Lip-S4D1-S3hybD1 (SEQ ID NO: 27) and Lip-S5D1-S6D1 (SEQ ID NO: 33). Preferably, the three heterodimers are mixed at a molar ratio of 1:2:1, 1:3:1, 1:1:2, 1:1:3, 1:2:2, 1:2:3, 1:3:2, 1:3:3, 2:1:1, 2:1:2, 2:1:3, 2:2:3, 2:2:1, 2:3:1, 2:3:2, 2:3:3, 3:1:1, 3:1:2, 3:1:3, 3:2:1, 3:2:2, 3:2:3, 3:3:1, 3:3:2, most preferably 1:1:1.

In a further embodiment, the pharmaceutical composition comprises two heterodimers, preferably Lip-S1D1-S2D1 (SEQ ID NO: 29) and Lip-S5D1-S6D1 (SEQ ID NO: 33), Lip-S1D1-S2D1 (SEQ ID NO: 29) and Lip-S4D1-S3hybD1 (SEQ ID NO: 27) or Lip-S4D1-S3hybD1 (SEQ ID NO: 27) and Lip-S5D1-S6D1 (SEQ ID NO: 33) in a molar ratio of 1:2, 1:3, 2:1, 3:1, 2:3, 3:2, preferably 1:1.

In one embodiment the pharmaceutical composition or vaccine of the invention further comprises at least one additional antigen from Borrelia or a pathogen other than Borrelia (herein referred to generically as “combination pharmaceutical composition or vaccine”). In a preferred embodiment, the at least one additional antigen is derived from a Borrelia species causing Lyme borreliosis. In various aspects, the at least one additional antigen is derived from another pathogen, preferably a tick-borne pathogen. In a further aspect, the pathogen causes Rocky Mountain spotted fever, Human granulocytic ehrlichiosis (HGE), Sennetsu Fever, Human Monocytic Ehrlichiosis (HME), Anaplasmosis, Boutonneuse fever, Rickettsia parkeri Rickettsiosis, Southern Tick-Associated Rash Illness (STARI), Helvetica Spotted fever, 364D Rickettsiosis, African spotted fever, Relapsing fever, Tularemia, Colorado tick fever, Tick-borne encephalitis (TBE, also known as FSME), Crimean-Congo hemorrhagic fever, Q fever, Omsk hemorrhagic fever, Kyasanur forest disease, Powassan encephalitis, Heartland virus disease or Babesiosis. In a further aspect, the disease is Japanese encephalitis.

In a further embodiment, the at least one additional antigen is derived from a vector-borne, preferably a tick-borne, pathogen selected from the group comprising Borrelia hermsii, Borrelia parkeri, Borrelia duttoni, Borrelia miyamotoi, Borrelia turicatae, Rickettsia rickettsii, Rickettsia australis, Rickettsia conori, Rickettsia helvetica, Francisella tularensis, Anaplasma phagocytophilum, Ehrlichia sennetsu, Ehrlichia chaffeensis, Neoehrlichia mikurensis, Coxiella burnetii and Borrelia lonestari, Tick-borne encephalitis virus (TBEV aka FSME virus), Colorado tick fever virus (CTFV), Crimean-Congo hemorrhagic fever virus (CCHFV), Omsk Hemorrhagic Fever virus (OHFV), Japanese encepalitis virus (JEV) and Babesia spp.

In another aspect, the invention relates to a kit comprising the pharmaceutical composition of the present invention and an additional antigen as defined above, wherein the at least one additional antigen is comprised in a second composition, particularly wherein the second composition is a vaccine, preferably a tick-borne encephalitis vaccine, a Japanese encephalitis vaccine or a Rocky Mountain spotted fever vaccine. The first composition may be a vaccine as well. The combination pharmaceutical composition or vaccine of the invention comprises any composition discussed herein in combination with at least a second (vaccine) composition. In some aspects, the second vaccine composition protects against a vector-borne disease, preferably a tick-borne disease. In various aspects, the second vaccine composition has a seasonal immunization schedule compatible with immunization against Borrelia infection or Lyme borreliosis. In other aspects, combination vaccines are useful in the prevention of multiple diseases for use in geographical locations where these diseases are prevalent.

In one aspect, the second composition is a vaccine selected from the group consisting of a tick-borne encephalitis vaccine, a Japanese encephalitis vaccine, and a Rocky Mountain Spotted Fever vaccine. In a preferred aspect, the vaccine composition is FSME-IMMUN® (Baxter), Encepur® (Novartis Vaccines), EnceVir® (Microgen NPO) or TBE Moscow Vaccine® (Chumakov Institute of Poliomyelitis and Viral Encephalitides of Russian Academy of Medical Sciences). In another preferred aspect, the vaccine composition is IXIARO®/JESPECT® (Valneva SE), JEEV® (Biological E, Ltd.) or IMOJEV® (Sanofi Pasteur).

There is further provided a pharmaceutical composition which is a vaccine, this vaccine may further comprise a pharmaceutically acceptable excipient. In a preferred embodiment, the excipient is L-methionine.

The invention also includes immunogenic compositions. In some aspects, an immunogenic composition of the invention comprises any of the compositions discussed herein and a pharmaceutically acceptable carrier. In various aspects, the immunogenic composition has the property of inducing production of an antibody that specifically binds an outer surface protein A (OspA) protein. In certain aspects, the immunogenic composition has the property of inducing production of an antibody that specifically binds Borrelia. In particular aspects, the immunogenic composition has the property of inducing production of an antibody that neutralizes Borrelia. In some aspects, the antibody is produced by an animal. In further aspects, the animal is a mammal. In even further aspects, the mammal is human.

The vaccine preparations containing pharmaceutical compositions of the present invention may be used to protect a mammal susceptible to Borrelia infection or treat a mammal with a Borrelia infection, by means of administering said vaccine via a systemic or mucosal route. These administrations may include injection via the intramuscular, intraperitoneal, intradermal or subcutaneous routes; or via mucosal administration to the oral/alimentary, respiratory or genitourinary tracts. Although the vaccine of the invention may be administered as a single dose, components thereof may also be co-administered together at the same time or at different times.

In one aspect of the invention is provided a vaccine kit, comprising a vial containing a pharmaceutical composition of the invention, optionally in lyophilised form, and further comprising a vial containing an adjuvant as described herein. It is envisioned that in this aspect of the invention, the adjuvant will be used to reconstitute the lyophilised immunogenic composition. In a further aspect, the pharmaceutical composition of the invention may be pre-mixed in a vial, preferably in a syringe.

A further aspect of the invention is a method of treating or preventing a Borrelia infection in a subject in need thereof comprising the step of administering to the subject a therapeutically-effective amount of a polypeptide of the present invention, the nucleic acid of the present invention, the antibody of the present invention or the pharmaceutical composition of the present invention.

A further aspect of the invention is a method of immunizing a subject in need thereof comprising the step of administering to the subject a therapeutically-effective amount of a polypeptide of the present invention, the nucleic acid of the present invention, the antibody of the present invention or the pharmaceutical composition of the present invention.

A still further aspect relates to a method for immunizing an animal or human against infection with a Borrelia organism, comprising the step of administering to said animal or human an effective amount a polypeptide of the present invention, the nucleic acid of the present invention, the antibody of the present invention or the pharmaceutical composition of the present invention, wherein the effective amount is suitable to elicit an immune response in said animal or human

Yet another aspect relates to a method for stimulating an immune response in an animal or human against a Borrelia organism, comprising the step of administering to said animal or human an effective amount of a polypeptide of the present invention, the nucleic acid of the present invention, the antibody of the present invention or the pharmaceutical composition of the present invention, wherein the effective amount is suitable to stimulate an immune response in said animal or human

Preferably, the Borrelia organism is selected from the group comprising B. burgdorferi, particularly B. burgdorferi s.s., B. garinii, B. afzelii, B. andersoni, B. bavariensis, B. bissettii, B. valaisiana, B. lusitaniae, B. spielmanii, B. japonica, B. tanukii, B. turdi or B. sinica, preferably B. burgdorferi s.s., B. afzelii or B. garinii.

In one embodiment there is provided a method of preventing or treating primary and/or recurring episodes of Borrelia infection comprising administering to the host an immunoprotective dose of the pharmaceutical composition or vaccine or kit of the invention.

A further aspect of the invention is a pharmaceutical composition of the invention for use in the treatment or prevention of Borrelial disease. In one embodiment there is provided a pharmaceutical composition for use in the treatment or prevention of Borrelia infection.

A further aspect of the invention is the use of the pharmaceutical composition or vaccine or kit of the invention in the manufacture of a medicament for the treatment or prevention of Borrelia infection. In one embodiment there is provided a pharmaceutical composition of the invention for use in the manufacture of a medicament for the treatment or prevention of Borrelia infection.

The invention also includes methods for inducing an immunological response in a subject. In various aspects, such methods comprise the step of administering any of the immunogenic compositions or vaccine compositions discussed herein to the subject in an amount effective to induce an immunological response. In certain aspects, the immunological response comprises production of an anti-OspA antibody.

The invention includes methods for preventing or treating a Borrelia infection or Lyme boreliosis in a subject. In various aspects, such methods comprise the step of administering any of the vaccine compositions discussed herein or any of the combination vaccines discussed herein to the subject in an amount effective to prevent or treat the Borrelia infection or Lyme borreliosis.

The invention includes uses of polypeptides, nucleic acids, antibodies, pharmaceutical compositions or vaccines of the invention for the preparation of medicaments. Other related aspects are also provided in the instant invention.

The terms “comprising”, “comprise” and “comprises” herein are intended by the inventors to be optionally substitutable with the terms “consisting of”, “consist of” and “consists of”, respectively, in every instance. The term “comprises” means “includes”. Thus, unless the context requires otherwise, the word “comprises”, and variations such as “comprise” and “comprising” will be understood to imply the inclusion of a stated compound or composition (e.g., nucleic acid, polypeptide, antibody) or step, or group of compounds or steps, but not to the exclusion of any other compounds, composition, steps, or groups thereof. The abbreviation, “e.g.” is derived from the Latin exempli gratia, and is used herein to indicate a non-limiting example. Thus, the abbreviation “e.g.” is synonymous with the term “for example”.

Embodiments herein relating to “vaccine compositions” of the invention are also applicable to embodiments relating to “pharmaceutical compositions” of the invention, and vice versa.

Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Definitions of common terms in molecular biology can be found in Benjamin Lewin, Genes V, published by Oxford University Press, 1994 (ISBN 0-19-854287-9); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology, published by Blackwell Science Ltd., 1994 (ISBN 0-632-02182-9); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995 (ISBN 1-56081-569-8).

The singular terms “a”, “an”, and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. The term “plurality” refers to two or more. It is further to be understood that all base sizes or amino acid sizes, and all molecular weight or molecular mass values given for nucleic acids or polypeptides are approximate, and are provided for description. Additionally, numerical limitations given with respect to concentrations or levels of a substance, such as an antigen, may be approximate.

A preferable carrier or excipient for the polypeptides according to the present invention in their diverse embodiments, or a nucleic acid molecule according to the present invention is an immunostimulatory compound such as an adjuvant for further stimulating the immune response to the polypeptide according to the present invention or a coding nucleic acid molecule thereof.

Adjuvants or immunostimulatory compounds may be used in compositions of the invention. Preferably, the immunostimulatory compound in pharmaceutical compositions according to the present invention is selected from the group of polycationic substances, especially polycationic peptides, immunostimulatory nucleic acids molecules, preferably immunostimulatory deoxynucleotides, oil-in-water or water-in-oil emulsions, MF59, aluminium salts, Freund's complete adjuvant, Freund's incomplete adjuvant, neuroactive compounds, especially human growth hormone, or combinations thereof.

The use of an aluminium hydroxide and/or aluminium phosphate adjuvant is particularly preferred, and antigens are generally adsorbed to these salts. Preferably, aluminium hydroxide is present at a final concentration of 0.15%. A useful aluminium phosphate adjuvant is amorphous aluminium hydroxyphosphate with PO₄/Al molar ratio between 0.84 and 0.92. Another adjuvant useful in the current invention is an aluminium salt that is able to provide an aqueous composition having less than 350 ppb heavy metal based on the weight of the aqueous composition. A further useful aluminium-based adjuvant is AS04, a combination of aluminium hydroxide and monophosphoryl lipid A (MPL).

Also, the pharmaceutical composition in accordance with the present invention is a pharmaceutical composition which comprises at least any of the following compounds or combinations thereof: the nucleic acid molecules according to the present invention, the polypeptides according to the present invention in their diverse embodiments, the vector according to the present invention, the cells according to the present invention and the antibody according to the present invention. In connection therewith, any of these compounds may be employed in combination with a non-sterile or sterile carrier or carriers for use with cells, tissues or organisms, such as a pharmaceutical carrier suitable for administration to a subject. Such carriers may include, but are not limited to, saline, buffered saline, dextrose, water, glycerol, ethanol and combinations thereof. The formulation should suit the mode of administration.

In one embodiment, the pharmaceutical composition comprises a stabilizer. The term “stabilizer” refers to a substance or vaccine excipient which protects the immunogenic composition of the vaccine from adverse conditions, such as those which occur during heating or freezing, and/or prolongs the stability or shelf-life of the immunogenic composition in a stable and immunogenic condition or state. Examples of stabilizers include, but are not limited to, sugars, such as sucrose, lactose and mannose; sugar alcohols, such as manitol; amino acids, such as glycine or glutamic acid; and proteins, such as human serum albumin or gelatin.

The pharmaceutical compositions of the present invention may be administered in any effective, convenient manner including, for instance, administration by topical, oral, anal, vaginal, intravenous, intraperitoneal, intramuscular, subcutaneous, intranasal, intratracheal or intradermal routes, among others. In a preferred embodiment, the pharmaceutical compositions are administered subcutaneously or intramuscularly, most preferably intramuscularly.

In therapy or as a prophylactic, the active agent of the pharmaceutical composition of the present invention may be administered to an individual as an injectable composition, for example as a sterile aqueous dispersion, preferably isotonic.

Alternatively the composition, preferably the pharmaceutical composition may be formulated for topical application, for example in the form of ointments, creams, lotions, eye ointments, eye drops, ear drops, mouthwash, impregnated dressings and sutures and aerosols, and may contain appropriate conventional additives, including, for example, preservatives, solvents to assist drug penetration, and emollients in ointments and creams. Such topical formulations may also contain compatible conventional carriers, for example cream or ointment bases, and ethanol or oleyl alcohol for lotions. Such carriers may constitute from about 1% to about 98% by weight of the formulation; more usually they will constitute up to about 80% by weight of the formulation.

In addition to the therapy described above, the compositions of this invention may be used generally as a wound treatment agent to prevent adhesion of bacteria to matrix proteins exposed in wound tissue and for prophylactic use in dental treatment as an alternative to, or in conjunction with, antibiotic prophylaxis.

In a preferred embodiment the pharmaceutical composition is a vaccine composition. Preferably, such vaccine composition is conveniently in injectable form. Conventional adjuvants may be employed to enhance the immune response. A suitable unit dose for vaccination with a protein antigen is for adults between 0.02 μg and 3 μg antigen per kg body weight and for children between 0.2 μg and 10 μg antigen per kg body weight, and such dose is preferably administered 1 to 3 times at intervals of 2 to 24 weeks.

At the indicated dose range, no adverse toxicological effects are expected with the compounds of the invention, which would preclude their administration to suitable individuals.

As an additional aspect, the invention includes kits which comprise one or more pharmaceutical formulations for administration to a subject packaged in a manner which facilitates their use for administration to subjects. In a preferred embodiment, the kits comprise the formulation in a final volume of 2 mL, more preferably in a final volume of 1 mL.

In a specific embodiment, the invention includes kits for producing a single dose administration unit. The kits, in various aspects, each contain both a first container having a dried protein and a second container having an aqueous formulation. Also included within the scope of this invention are kits containing single and multi-chambered pre-filled syringes (e.g., liquid syringes and lyosyringes).

In another embodiment, such a kit includes a pharmaceutical formulation described herein (e.g., a composition comprising a therapeutic protein or peptide), packaged in a container such as a sealed bottle or vessel, with a label affixed to the container or included in the package that describes use of the compound or composition in practicing the method. In one embodiment, the pharmaceutical formulation is packaged in the container such that the amount of headspace in the container (e.g., the amount of air between the liquid formulation and the top of the container) is very small Preferably, the amount of headspace is negligible (i.e., almost none).

In one aspect, the kit contains a first container having a therapeutic protein or peptide composition and a second container having a physiologically acceptable reconstitution solution for the composition. In one aspect, the pharmaceutical formulation is packaged in a unit dosage form. The kit optionally further includes a device suitable for administering the pharmaceutical formulation according to a specific route of administration. In some aspects, the kit contains a label that describes use of the pharmaceutical formulations.

The pharmaceutical composition can contain a range of different antigens. Examples of antigens are whole-killed or attenuated organisms, subfractions of these organisms, proteins, or, in their most simple form, peptides. Antigens can also be recognized by the immune system in the form of glycosylated proteins or peptides and may also be or contain polysaccharides or lipids. Short peptides can be used, since cytotoxic T cells (CTL) recognize antigens in the form of short, usually 8-11 amino acid long, peptides in conjunction with major histocompatibility complex (MHC). B cells can recognize linear epitopes as short as 4 to 5 amino acids, as well as three-dimensional structures (conformational epitopes).

In a preferred embodiment, the pharmaceutical composition of another aspect additionally comprises a hyperimmune serum-reactive antigen against a Borrelia protein or an active fragment or variant thereof, such as, e.g., the antigens, fragments and variants as described in WO2008/031133.

According to the invention, the pharmaceutical composition according to another aspect may be used as a medicament, particularly as a vaccine, particularly in connection with a disease or disease condition which is caused by, linked or associated with Borrelia.

The pharmaceutical composition of the present invention may be used as a medicament, particularly as a vaccine, particularly in connection with a disease or disease condition which is caused by, linked with or associated with Borrelia, more preferably any pathogenic Borrelia species and more preferably in a method for treating or preventing a Borrelia infection, particularly a B. burgdorferi s.s., B. garinii, B. afzelii, B. andersoni, B. bavariensis, B. bissettii, B. valaisiana, B. lusitaniae, B. spielmanii, B. japonica, B. tanukii, B. turdi or B. sinica infection, preferably a B. burgdorferi s.s., B. afzelii or B. garinii infection.

In connection therewith, it should be noted that the various Borrelia species, including B. burgdorferi s.l., comprise several species and strains including those disclosed herein. A disease related, caused or associated with the bacterial infection to be prevented and/or treated according to the present invention includes Lyme borreliosis (Lyme disease). Further aspects, symptoms, stages and subgroups of Lyme borreliosis as well as specific groups of patients suffering from such disease as also disclosed herein, including in the introductory part, are incorporated herein by reference. More specifically, Lyme borreliosis generally occurs in stages, with remission and exacerbations with different clinical manifestation at each stage. Early infection stage 1 consists of localized infection of the skin, followed within days or weeks by stage 2, disseminated infection, and months to years later by stage 3, persistent infection. However, the infection is variable; some patients have only localized infections of the skin, while others display only later manifestations of the illness, such as arthritis.

In a fourth aspect, the present invention relates to a method of treating or preventing a Borrelia infection in a subject in need thereof, comprising the step of administering to the subject a therapeutically effective amount of a pharmaceutical composition according to the third aspect.

The term “subject” is used throughout the specification to describe an animal, preferably a mammal, more preferably a human, to whom a treatment or a method according to the present invention is provided. For treatment of those infections, conditions or disease states which are specific for a specific animal such as a human patient, the term patient refers to that specific animal Preferably, the subject is a human; however, the medical use of the composition may also include animals such as poultry including chicken, turkey, duck or goose, livestock such as horse, cow or sheep, or companion animals such as dogs or cats.

The term “effective amount” is used throughout the specification to describe an amount of the present pharmaceutical composition which may be used to induce an intended result when used in the method of the present invention. In numerous aspects of the present invention, the term effective amount is used in conjunction with the treatment or prevention. In other aspects, the term effective amount simply refers to an amount of an agent which produces a result which is seen as being beneficial or useful, including in methods according to the present invention where the treatment or prevention of a Borrelia infection is sought.

The term effective amount with respect to the presently described compounds and compositions is used throughout the specification to describe that amount of the compound according to the present invention which is administered to a mammalian patient, especially including a human patient, suffering from a Borrelia-associated disease, to reduce or inhibit a Borrelia infection.

In a preferred embodiment, the method of immunizing a subject according to the above aspect comprises the step of administering to the subject a therapeutically effective amount of a pharmaceutical composition of the third aspect of the current invention.

The method comprises inducing an immunological response in an individual through gene therapy or otherwise, by administering a polypeptide or nucleic acid according to the present invention in vivo in order to stimulate an immunological response to produce antibodies or a cell-mediated T cell response, either cytokine-producing T cells or cytotoxic T cells, to protect said individual from disease, whether or not that disease is already established within the individual.

The products of the present invention, particularly the polypeptides and nucleic acids, are preferably provided in isolated form, and may be purified to homogeneity. The term “isolated” as used herein means separated “by the hand of man” from its natural state; i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a naturally-occurring nucleic acid molecule or a polypeptide naturally present in a living organism in its natural state is not “isolated”, but the same nucleic acid molecule or polypeptide separated from the coexisting materials of its natural state is “isolated”, as the term is employed herein. As part of or following isolation, such nucleic acid molecules can be joined to other nucleic acid molecules, such as DNA molecules, for mutagenesis, to form fusion genes, and for propagation or expression in a host, for instance. The isolated nucleic acid molecules, alone or joined to other nucleic acid molecules such as vectors, can be introduced into host cells, in culture or in whole organisms. Introduced into host cells in culture or in whole organisms, such DNA molecules still would be isolated, as the term is used herein, because they would not be in their naturally-occurring form or environment. Similarly, the nucleic acid molecules and polypeptides may occur in a composition, such as medium formulations, solutions for introduction of nucleic acid molecules or polypeptides, for example, into cells, compositions or solutions for chemical or enzymatic reactions, for instance, which are not naturally occurring compositions, and, therein remain isolated nucleic acid molecules or polypeptides within the meaning of that term as it is employed herein.

The invention is not limited to the particular methodology, protocols and reagents described herein because they may vary. Furthermore, the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention. As used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural reference unless the context clearly dictates otherwise. Similarly, the words “comprise”, “contain” and “encompass” are to be interpreted inclusively rather than exclusively.

Unless defined otherwise, all technical and scientific terms and any acronyms used herein have the same meanings as commonly understood by one of ordinary skill in the art in the field of the invention. Although any methods and materials similar or equivalent to those described herein can be used in the practice of the present invention, the preferred methods, and materials are described herein.

The present invention is further illustrated by the following Figures, Tables, Examples and the Sequence listing, from which further features, embodiments and advantages may be taken. As such, the specific modifications discussed are not to be construed as limitations on the scope of the invention. It will be apparent to the person skilled in the art that various equivalents, changes, and modifications may be made without departing from the scope of the invention, and it is thus to be understood that such equivalent embodiments are to be included herein.

In connection with the present invention

FIG. 1 shows electrostatic potential isocontours of disulfide-bond-stabilized fragments of serotype 1-serotype 6 and B. valaisiana OspA (S1D1-S6D1 and BvaD1), as well as the mutant fusion OspA fragment of the invention consisting of amino acids 125-176 of B. valaisiana, strain VS116, and amino acids 177-274 of B. garinii, strain PBr, with an introduced disulfide bond (S3hybD1).

FIG. 2 shows the amino acid alignment of Lip-S4D1-S3hybD1 (SEQ ID NO: 27), the mutant serotype 3 OspA fusion fragment-containing heterodimer protein of the invention, with the heterodimer protein Lip-S4D1-S3D1 (SEQ ID NO: 31).

FIG. 3 schematically shows the production of mutant OspA fragment heterodimers according to the current invention.

FIG. 4 schematically represents the polypeptide components of a pharmaceutical composition of the current invention, an “improved combination vaccine”, comprising three different mutant OspA heterodimers, including Lip-S4D1-S3hybD1.

FIG. 5 shows the chemical structure of Pam₃Cys, an example of a fatty acid substituted cysteine, such as would be found at the N-terminus of lipidated polypeptides of the current invention.

FIG. 6 shows IgG antibody titers to Borrelia OspA proteins of serotype 1-6 produced in mice in response to immunization with the improved heterodimer combination vaccine of the invention.

FIG. 7 shows the binding of antibodies from mice immunized with the improved heterodimer combination vaccine of the invention to the cell surface of Borrelia spirochetes of OspA serotypes 1-6.

Table 1 compares the purification yield of the Lip-S4D1-S3hybD1 heterodimer and the Lip-S4D1-S3D1 heterodimer.

Table 2 shows the protective capacity of the improved heterodimer combination vaccine of the invention against in vivo challenge with OspA serotypes 1, 2, 5 and 6 Borrelia.

The figures and tables which may be referred to in the specification are described below in more detail.

FIG. 1 Electrostatic potential simulation of the disulfide-bond-stabilized OspA fragments from serotypes 1-6 (S1D1-S6D1) and B. valaisiana (BvaD1) as well as the disulfide-bond-stabilized fusion OspA fragment (S3hybD1). Isocontours (+/−1 kT/e) are colored bright for negative charges and dark for positive charges, as a solid surface. The solvent-accessible surface is rendered as a wireframe. The white arrows indicate the position of the two extended clusters giving rise to electrostatic polarity on the same plane of the serotype 3 fragment. It can be seen that the surfaces of the hybrid OspA C-terminal fragment do not possess the extended electrostatic polar clusters as the S3D1 monomer.

FIG. 2 Amino acid sequence alignment of Lip-S4D1-S3hybD1 (SEQ ID NO: 27) and Lip-S4D1-S3D1 (SEQ ID NO: 31) heterodimer polypeptides, showing the consensus sequence. The Lip-S4D1-S3hybD1 heterodimer differs from the Lip-S4D1-S3D1 heterodimer by only 31 amino acids in total.

FIG. 3 Production of a mutant OspA heterodimer of the invention comprising two mutant OspA C-terminal fragments selected from different OspA serotypes of Borrelia sp. or a hybrid mutant OspA C-terminal fragment (A) Schematic representation of a nucleic acid encoding a lipidated mutant OspA heterodimer. The components, from 5′ to 3′, comprise the coding sequences for a lipidation signal sequence (Lip signal), a CSS peptide for N-terminal lipidation, a mutant C-terminal fragment of OspA with two non-native cysteines, a short linker peptide (LN1), followed by a second mutant OspA C-terminal fragment with two non-native cysteines. (B) The intermediate mutant OspA heterodimer polypeptide comprises the nascent product directly following translation of the nucleic acid construct. From the N- to the C-terminus, this polypeptide consists of a lipidation signal sequence (Lip signal), a CSS peptide for lipidation, a mutant OspA fragment with a non-native disulfide bond, a short linker peptide (LN1), followed by a second mutant OspA fragment with a non-native disulfide bond. (C) The final lipidated mutant OspA heterodimer polypeptide after post-translational modification. The heterodimer, from the N- to the C-terminus, consists of a CSS peptide with the N-terminal cysteine lipidated, a mutant OspA fragment stabilized by a disulfide bond, a linker peptide (LN1), and a second mutant OspA fragment stabilized by a disulfide bond. The lipidation signal sequence is cleaved off during post-translational modification of the polypeptide as shown.

FIG. 4 An example of a preferred pharmaceutical composition according to the current invention. Three mutant OspA heterodimers, each comprising two mutated OspA fragments selected from different Borrelia OspA serotypes or a hybrid mutant OspA C-terminal fragment (S3hybD1) are present in the composition, together providing OspA antigens from six different Borrelia OspA serotypes. Such a pharmaceutical composition enables simultaneous immunization against six Borrelia serotypes.

FIG. 5 Illustration of the chemical structure of Pam₃Cys, an example of a fatty acid substitution of the N-terminal cysteine of full-length wild-type OspA protein as well as of lipidated mutant OspA fragment heterodimers of the invention. During post-translational modification of a full-length OspA protein or polypeptides of the invention, the N-terminal lipidation signal sequence is cleaved off and fatty acids, most commonly three palmitoyl moieties (“Pam₃”), are enzymatically covalently attached to the N-terminal cysteine residue (the sulfur atom, “S”, is indicated by an arrow). The remaining residues of the polypeptide chain, which are located C-terminally from the Pam₃Cys residue, are represented by “Xn”. (Modified from Bouchon, et al. (1997) Analytical Biochemistry 246: 52-61.)

FIG. 6 Antibody titers generated to all six serotypes of full-length OspA proteins. Mice were immunized three times with 3 μg each of the indicated combination vaccines: Lip-S1D1-S2D1, Lip-54D1-53D1 and Lip-S5D1-S6D1 together in a 1:1:1 ratio (“Het combo”); Lip-S1D1-S2D1, Lip-54D1-S3hybD1 and Lip-S5D1-S6D1 together in a 1:1:1 ratio (“Improved het combo”) or with Lip-Chimeric OspA ST1/ST2-His, Lip-Chimeric OspA ST5/ST3-His and Lip-Chimeric OspA ST6/ST4-His together in a 1:1:1 ratio (“Chimera combo”) at two week intervals and sera were collected at one week after the last dose. Titers of IgG antibodies to six different serotypes of full-length OspA proteins were determined by ELISA.

FIG. 7 Binding of antibodies from immunized mice to the cell surface of Borrelia spirochetes. Mice were immunized as above and sera were collected at one week after the last dose and pooled. Serial dilutions of the sera were tested for binding to the cell surface of Borrelia spirochetes via cell staining and flow cytometry. Fluorescence intensity values observed when staining with sera collected from control mice immunized with Al(OH)₃ adjuvant alone were subtracted to account for non-specific binding. (Borrelia used in the binding assay were: B. burgdorferi, OspA serotype 1, strain N40; B. afzelii, OspA serotype 2, strain PKo; B. garinii, OspA serotype 3, strain Fr; B. bavariensis, OspA serotype 4, strain Fin; B. garinii, OspA serotype 5, strain PHei; B. garinii, OspA serotype 6, strain KL11.)

EXAMPLES Example 1. Molecular Modelling of the Hybrid Serotype 3 OspA C-Terminal Fragment

Motivation to Construct Hybrid OspA ST3 C-Terminal Fragments

The Lyme borreliosis combination vaccine as described in our previous application (WO2014/006226) is composed of three mutant OspA heterodimers. Short stabilized fragments from two different OspA serotypes (ST), derived from the C-terminal domain, are fused with a short linker to form a heterodimer. The three heterodimers are composed of OspA ST1-ST2, ST4-ST3 and ST5-ST6. For improvement of the immunogenicity of the heterodimers, a signal sequence for lipidation is added in analogy with mature full-length OspA which is a lipoprotein.

The lipidated heterodimer composed of OspA ST4-ST3 (Lip-S4D1-S3D1; SEQ ID NO: 31) proved to be less soluble than the two other heterodimers, which results in low recovery during purification. This problem can mostly be attributed to the short stabilized OspA ST3 portion since this protein, as a lipidated monomer, cannot be expressed and purified.

Molecular Modelling

A comparative structural investigation of the stabilized monomers was untertaken in silico to elucidate the compatibility of the folds between the monomer models compared to the OspA crystal structure (PDB:1OSP; Li H, Dunn J J, Luft B J, Lawson C L (1997) Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab. Proc Natl Acad Sci 94: 3584-3589) and to compare their surface properties with the ST3 type monomer. The crystal structure represents serotype 1 of Borrelia burgdorferi B31, and shows OspA bound with its N-terminus, to the murine antibody Fab 184.1. Homology structure models of the six short stabilized OspA monomers were constructed starting with the ST1 OspA crystal structure and available homology models (SwissModel; Kiefer F, Arnold K, Kunzli M, Bordoli L, Schwede T (2009) The SWISS-MODEL Repository and associated resources. Nucleic Acids Res 37: D387-392), which were then modified to incorporate the stabilizing disulfide bonds and sequence changes where applicable (The PyMOL Molecular Graphics System, Version Open-Source, Schrödinger LLC).

The electrostatic potential isocontours of all six short stabilized OspA monomers were simulated with the adaptive Poisson-Boltzmann solver (APBS; Baker N A, Sept D, Joseph S, Holst M J, McCammon J A (2001) Electrostatics of nanosystems: application to microtubules and the ribosome. Proc Natl Acad Sci 98: 10037-10041, pdb2pqr; Dolinsky T J, Czodrowski P, Li H, Nielsen J E, Jensen J H, et al. (2007) PDB2PQR: expanding and upgrading automated preparation of biomolecular structures for molecular simulations. Nucleic Acids Res 35: W522-525) to determine if the short stabilized OspA ST3 protein has a pattern in surface electrostatics which deviates from the other OspA STs, which could explain the solubility problem of short stabilized OspA ST3 (“S3D1”, FIG. 1 ). A significant polarity in the charge distribution was observed on one side of S3D1 (see arrows), which was not observed in any of the other short stabilized OspA STs.

An electrostatic potential simulation was also performed with short stabilized OspA from B. valaisiana (“BvaD1”, FIG. 1 ). The BvaD1 OspA fragment displayed a variant polarity of the electrostatic potential isocontours on the surface, which made it a potential candidate building block for a partial segmental exchange with the aim to modify the overall surface to not show any significant extended clusters of extended electrostatic polarity as found in S3D1. The preferred link between the serotype 3 part and the exchanged part from B. valaisiana in the hybrid monomer was chosen to replace the N-terminal beta-sheet of the monomer and to leave two beta sheets and the C-terminal helix of the serotype 3 portion intact, under retention of the overall fold. The latter condition depends largely on the steric compatibility of the densely packed hydrophobic residues in the core of the molecule. Fold compatibility for the model of the hybrid stabilized monomer, S3hybD1, was verified with molecular mechanics simulation (Gromacs; Pronk S, Pall S, Schulz R, Larsson P, Bjelkmar P, et al. (2013) GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit. Bioinformatics 29: 845-854).

Application in the Form of Heterodimers Containing the Hybrid OspA Fragment

As a result of the electrostatic potential simulations, a new heterodimer containing the experimental fusion of fragments OspA proteins from B. valaisiana and B. garinii (S3hybD1, FIG. 1 ), was cloned: Lip-S4D1-S3hybD1. In addition to the changes of the first one-third of the serotype 3 OspA fragment in the heterodimer, 54D1-S3hybD1, compared with Lip S4D1-S3D1, an amino acid substitution at position 233 (P233T, amino acid nomenclature according the immature full-length OspA; SEQ ID NO: 8) of OspA ST3 was introduced.

As illustrated in further detail below, this new heterodimer shows substantially improved solubility as well as immunogenicity against Borrelia expressing serotype 3 OspA when purified and used to immunize mice.

Example 2. Purification and Formulation of Lipidated Mutant OspA Fragment Heterodimers

Cloning and Expression of Lipidated Non-His-Tagged Mutant OspA Fragment Heterodimers

The fusion OspA monomer B. valaisiana/B. garinii strain VS116/PBr was codon-optimized for E. coli expression by GeneArt (Germany) The lipidation signal sequence added to the N-terminal end was derived from the E. coli major outer membrane lipoprotein, Lpp, and was followed directly C-terminally by a CSS peptide to provide an N-terminal cysteine for lipidation. The improved heterodimer construct was generated by fusing the mutant serotype 4 OspA fragment and the hybrid serotype 3 OspA fragment via the linker sequence “LN1”. Gene fragments were cloned into the pET28b(+) vector (Novagen, USA), and the stabilized heterodimers were expressed in BL21(DE3) cells (Invitrogen, USA). Cells were collected after 4 h by centrifugation and the pellet was stored at −70° C. for up to 12 months prior to further processing.

Purification of Lipidated Mutant OspA Fragment Heterodimers

Cells were disrupted mechanically by high-pressure homogenization and the lipidated mutant OspA fragment heterodimers, Lip-S4D1-S3D1 and Lip-S4D1-S3hybD1, were enriched in the lipid phase by phase separation, using Triton X-114 as detergent. Subsequently, the diluted detergent phase was subjected to anion exchange chromatography (Q-sepharose; GE Healthcare, United Kingdom) operated in non-binding mode. The resulting flow-through was loaded on a hydroxyapatite column (Bio-Rad, USA) and the lipidated proteins were eluted from the column by a linear salt gradient. The eluate was subjected to further purification over a DEAE-Sepharose column (GE Healthcare) in non-binding mode followed by gel filtration column (Superdex 200, GE Healthcare) for buffer exchange. The lipidated mutant OspA heterodimer peaks were pooled on the basis of the analytical size exclusion column and SDS-PAGE. After sterile filtration, the purified heterodimers were stored at −20° C. until formulation.

Formulation of the Combination Vaccine

Studies regarding the formulation of the combination vaccine of the invention were carried out in order to optimize stability. Different types of buffers and stabilizers were tested at various concentrations in combination with aluminium hydroxide and antigen, as described in our previous application WO2014/006226. An optimal formulation of 40 μg/mL each of three heterodimers (120 μg total protein), 10 mM sodium phosphate, 150 mM sodium chloride, 10 mM L-Methionine, 5% Sucrose, 0.05% Tween 20 (polysorbate 20) and 0.15% (w/v) aluminium hydroxide at pH 6.7±0.2 was determined.

Results

The improved heterodimer, Lip-S4D1-S3hybD1 showed an about 4-fold higher yield in terms of mg/g biomass, a significant improvement over Lip-S4D1-S3D1. Additionally, comparable purity of the improved heterodimer preparation was achievable with one less chromatography step. (See Table 1.)

TABLE 1 Improved yield of heterodimer Lip S4D1-S3hybD1 compared with Lip-S4D1-S3D1. Number of Yield (mg/g Purity (%) Purity (%) Purity (%) Chromatography Construct biomass) RP-HPLC SEC-HPLC SDS-PAGE steps Lip-S4D1-S3D1 0.35 88 95 90 5 Lip-S4D1-S3hybD1 2.5 82 96 97 4

Example 3. Immunogenicity of Lipidated Mutant OspA Fragment Heterodimers of Different Serotypes

Immunization of mice Female C₃H/HeN mice were used for all studies. Prior to immunizations, groups of ten mice were bled via the facial vein and pre-immune sera were prepared and pooled. Three s c immunizations of 100 μL each were administered at two week intervals. Each dose contained 1 μg of the respective heterodimer proteins. For the improved heterodimer combination vaccine: Lip-S1D1-S2D1 (SEQ ID NO: 29), Lip-S4D1-S3hybD1 (SEQ ID NO: 27) and Lip-S5D1-S6D1 (SEQ ID NO: 33); for the heterodimer combination vaccine of the previous invention: Lip-S1D1-S2D1 (SEQ ID NO: 29), Lip-54D1-53D1 (SEQ ID NO: 31) and Lip-S5D1-S6D1 (SEQ ID NO: 33) and for the chimera combination vaccine: Lip-Chimeric OspA ST1/ST2-His (Seq ID No: 40), Lip-Chimeric OspA ST5/ST3-His (Seq ID No: 41) and Lip-Chimeric OspA ST6/ST4-His (SEQ ID NO: 42). All combination vaccines were formulated with aluminium hydroxide (Al(OH)₃) at a final concentration of 0.15%. One week after the third immunization, blood was collected from the facial vein and immune sera prepared. In each experiment, one group immunized with PBS formulated with Al(OH)₃ was included as a negative control (placebo group). All animal experiments were conducted in accordance with Austrian law (BGB1 Nr. 501/1989) and approved by “Magistratsabteilung 58”.

OspA ELISA

ELISA plates (Maxisorp, Nunc, Denmark) were coated with 50 ng (1 μg/mL) protein diluted in coating buffer (PBS) per well and incubated at 4° C. for 16 to 72 hours. The coating antigens were C-terminally His-tagged full length lipidated OspA ST1-6. The coating buffer was discarded and 100 μL blocking buffer (1% BSA, 0.5% Tween-20, PBS) was added and incubated at ambient temperature for 1-2 hours. Plates were washed three times with 300 μL (overflow) PBST (0.1% Tween-20, PBS). Five-fold dilutions of the sera were made in blocking buffer and 50 μL were added to each well and incubated for 1 hour at ambient temperature. Plates were washed three times with 300 μL (overflow) PBST. The secondary antibody (horseradish peroxidase [HRP]-conjugated rabbit anti-mouse IgG, DAKO, Denmark) was diluted 1:2000 in blocking buffer and 50 μL was added to each well and incubated for 1 hour at ambient temperature. Plates were washed three times with 300 μL (overflow) PBST. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Sigma-Aldrich, USA) was used as substrate for HRP, 50 μL of ABTS was added to each well and incubated for 15 minutes in the dark at ambient temperature. The reaction was stopped by the addition of 50 μL 1% SDS and the absorbance was read at 405 nm. A plate was regarded as valid when the absorbance of the blank was below 0.1. A sample was valid when the lowest dilution had an absorbance above 1.0 and the highest dilution was below 0.1. When these criteria were met, the half-max titer was determined. The half-max titer is the reciprocal of the dilution that corresponds to the mean absorbance between the highest and lowest dilutions.

Flow Cytometry

Spirochetes (1×10⁶) were mixed with an equal volume of 4% paraformaldehyde and incubated for 2 hours at room temperature in a 96-well plate (Nunclon 96U, Nunc). The plate was centrifuged for 5 minutes at 2,000 g and the supernatant was discarded. Cells were washed with 150 μL HBSS with 2% BSA (HBSS-B), centrifuged as above, and the supernatant was discarded. Mouse sera were heat inactivated by incubating them at 56° C. for 35 minutes. Heat inactivated sera were diluted in HBSS-B and sterile filtered by centrifuging at 4,000 g for 3 minutes using Costar spin-X centrifuge tube filters (0.22 μm, Corning, USA). Spirochetes were dissolved in 100 μL serum and incubated for 45 minutes at room temperature. The plate was centrifuged for 15 minutes at 2,000 g and the supernatant was discarded. The cells were washed once with 150 μL HBSS-B and then resuspended in 100 μL HBSS-B. One microliter secondary antibody (PE conjugated goat anti-mouse IgG, Beckman Coulter, USA) was added to the cells and incubated at room temperature for 45 minutes in the dark. Spirochetes were washed once with 150 μL HBSS-B and then resuspended in 200 μL HBSS containing 2.5 μM SYTO-17 DNA dye and incubated for 10 minutes at room temperature in the dark. The stained spirochetes were pelleted by centrifuging for 5 minutes at 2,000 g and subsequently resuspended in 200 μL HBSS. Labelled spirochetes were measured with an FC500 (Beckman Coulter) flow cytometer, gated for SYTO-17 positive events.

Results

Two different OspA heterodimer formulations (“het combo” and “improved het combo”) as well as an OspA chimera combination (“chimera combo”) were tested for immunogenicity in mice. Hyperimmune sera were analysed by ELISA for reactivity against full-length OspA (coating antigen) as well as for surface binding to Borrelia strains expressing different OspA serotypes (ST1 to ST6).

The ELISA results indicated that all vaccine combinations stimulated antibody responses to all six OspA serotypes (see FIG. 6 ). It is especially noteworthy with regard to the current invention that the improved OspA heterodimer combination vaccine resulted in higher levels of antibodies specific to serotype 3 OspA in comparison with the OspA heterodimer combination vaccine of the previous invention, whereas antibody levels to other OspA serotypes were comparably stimulated by both vaccines.

Binding of antibodies from hyperimmune mouse sera directly to borrelia spirochetes was observed in the case of Borreliae expressing all six OspA serotypes (see FIG. 7 ), indicating that the antibodies generated in response to all of the antigens are functionally active and can bind native OspA in situ. The fluorescence intensity was linear over a large range of serum dilutions. The fluorescence intensity observed in response to the improved heterodimer combination vaccine to spirochetes was comparable to those observed in response to the heterodimer combination vaccine and the chimera combination vaccine. Notably, with regard to binding to serotype 3 OspA borrelia, the antibodies generated by immunization with the improved vaccine were superior to antibodies generation in response to both of the other combination vaccines.

Example 4. Protective Capacity of the Improved Heterodimer Combination Vaccine Against In Vivo Borrelia Challenge

Immunization of Mice

Female C₃H/HeN (H-2^(k)) mice were used for all studies (Janvier, France). Prior to each challenge, groups of five 8-week-old mice were bled via the tail vein and pre-immune sera were prepared and pooled. Three subcutaneous (s c) immunizations of 100 μL were administered at two week intervals at the doses indicated in Table 2. Both the improved heterodimer combination vaccine and the chimera combination vaccine included three proteins at a ratio of 1:1:1 as described in Example 3. All formulations included aluminium hydroxide (Al(OH)₃) at a final concentration of 0.15%. One week after the third immunization, blood was collected and hyper-immune sera were prepared. In each experiment, one group injected with Al(OH)₃ alone (in formulation buffer or PBS) was included as a negative control and one group of mice immunized with the wild-type full-length lipidated OspA protein from the appropriate OspA serotype served as a positive control group (B. burgdorferi strain B31 (OspA serotype 1, SEQ ID NO: 34), B. afzelii strain K78 (OspA serotype 2, SEQ ID NO: 35), B. garinii strain PHei (OspA serotype 5, SEQ ID NO: 38) or B. garinii strain DK29 (OspA serotype 6, SEQ ID NO: 39)). All animal experiments were conducted in accordance with Austrian law (BGB1 Nr. 501/1989) and approved by “Magistratsabteilung 58”.

Needle Challenge of Immunized Mice with In Vitro Grown Borrelia

Two weeks after the last immunization, mice were challenged s.c. with spirochetes diluted in 100 μL growth medium (BSKII). B. burgdorferi strain ZS7 expressing OspA serotype 1 (experiments 1 and 2), B. garinii strain PHei expressing OspA serotype 5 (experiments 10 to 13) or B. garinii strain Ma expressing OspA serotype 6 (experiments 14 to 17) were used for challenge. The challenge doses were strain-dependent and dependent on the virulence of the individual strains, which was assessed by challenge experiments for determination of ID₅₀. Doses employed for needle challenge experiments ranged from 20 to 50 times the ID₅₀. Prior to each challenge, OspA expression was verified by flow cytometry (see example 3). Challenge of mice was only performed with cultures where >80% of cells were positive for OspA expression.

Challenge of Immunized Mice with Ticks Infected with B. burgdorferi or B. afzelii (“Tick Challenge”)

Two weeks after the last immunization, mice were challenged with ticks harboring B. burgdorferi strain Pra4 expressing OspA serotype 1 (experiment 3), B. burgdorferi strain Praa1 expressing OspA serotype 1 (experiments 4 and 5) or B. afzelii expressing OspA serotype 2 (experiments 6 to 9). In order to facilitate tick infection of the immunized mice, the hair of the back of each mouse was removed with Veet® Cream (Reckitt Benckiser, United Kingdom) and a small ventilated container was glued to the skin with super glue (Pattex, Germany) Thereafter, two to three I. ricinus nymphs infected with B. burgdorferi strain Pra1 or Pra 4 or B. afzelii strain IS1 were applied per mouse and allowed to attach and feed until they were fully engorged and dropped off. The feeding status was monitored daily for each individual tick. Only those mice from which at least one fully- or almost fully-fed tick was collected were included in the final readout.

Sacrifice of Mice and Collection of Material

Four or six weeks after needle or tick challenge, respectively, mice were sacrificed by cervical dislocation. Blood was collected by orbital bleeding and final sera were prepared and used for VlsE ELISA and/or western blot to determine infection status. In addition, the urinary bladder from each mouse was collected and DNA was extracted and subjected to quantitative PCR (qPCR) for identification of Borrelia.

ELISA with the Invariable Region 6 (IR6) of VlsE

A biotinylated 25-mer peptide (MKKDDQIAAAMVLRGMAKDGQFALK, SEQ ID NO: 59) derived from the sequence of B. garinii strain IP90 was used for the analysis (Liang F T, Alvarez A L, Gu Y, Nowling J M, Ramamoorthy R, Philipp M T. An immunodominant conserved region within the variable domain of VlsE, the variable surface antigen of Borrelia burgdorferi. J Immunol. 1999; 163:5566-73). Streptavidin pre-coated 96-well ELISA plates (Nunc), were coated with 100 μL/well (1 μg/mL) peptide in PBS supplemented with 0.1% Tween (PBS/0.1T). The plates were incubated overnight at 4° C. After coating with the peptide, the plates were washed once with PBS/0.1T. The plates were then blocked for one hour at room temperature (RT) with 100 μL/well of PBS+2% BSA, before being washed again with PBS/0.1T. Reactivity of post-challenge sera (final sera) to the peptide was tested at 1:200 and 1:400 dilutions in PBS+1% BSA. Plates were incubated for 90 minutes at RT before being washed three times with PBS/0.1T. Each well then received 50 μL of 1.3 μg/mL polyclonal rabbit anti-mouse IgG conjugated to HRP (Dako) in PBS+1% BSA. The plates were then incubated for 1 hour at RT. After three washes with PBS/0.1T, ABTS (50 μL/well) was added as substrate (Sigma-Aldrich) and color was allowed to develop for 30 minutes. Absorbance was measured at 405 nm. All sera were tested in duplicate. Negative controls included PBS instead of sera as well as plates not coated with the peptide. Sera from mice shown to be culture positive for borrelia infection were used as positive controls.

DNA Extraction and Purification

The urinary bladder from each mouse was subjected to DNA extraction and purification using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer's instructions with the following modification. Each urinary bladder was digested overnight at 60° C. using 100 recombinant Proteinase K (PCR grade; 14-22 mg/mL, Roche). The DNA was eluted in 50 μL sterile deionized water and stored at −20° C. As a negative control, every tenth sample was followed by one empty purification column in each DNA extraction and purification.

qPCR Targeting recA

Oligonucleotide primers were designed for the recA gene in a manner that they could be used in qPCR for identification of all relevant Borrelia species causing Lyme borreliosis (forward: CATGCTCTTGATCCTGTTTA, SEQ ID NO: 57, reverse: CCCATTTCTCCATCTATCTC, SEQ ID NO: 58). The recA fragment was cloned from the B. burgdorferi s.s. strain N40 into pET28b(+), to be used as standard in each reaction. The chromosomal DNA extracted from mouse urinary bladders was diluted 1:4 in water in order to reduce matrix effects observed with undiluted DNA. A master mix consisting of 10 μL SSoAdvanced™ SYBR® Green Supermix, 0.3 μL of each primer (10 μM), and 7.4 μL water was prepared for each experiment. Eighteen μL of master mix was mixed with 2 μL of the diluted DNA extracted from urinary bladder in micro-titer plates and the DNA was amplified using a CFX96 real-time PCR detection system (Bio-Rad). The DNA was denatured for 3 minutes at 95° C., followed by 50 cycles of 15 seconds at 95° C. and 30 seconds at 55° C. After amplification, the DNA was prepared for melting curve analysis by denaturation for 30 seconds at 95° C. followed by 2 minutes at 55° C. The melting curve analysis was performed by 5 seconds incubation at 55° C., with a 0.5° C. increase per cycle, and 5 seconds at 95° C. On each plate, four no-template controls (NTC) were included as well as a standard curve in duplicate with template copy numbers ranging from 10 to 10,000.

Western Blot

Binding of final sera to whole cell lysates from borrelia belonging to the corresponding OspA serotype was analyzed by western blot. Briefly, 2.5 μg of spirochete lysate per mouse sera to be analyzed was separated by SDS-PAGE under reducing conditions using 4-12% Tris-Glycine ZOOM gels (Invitrogen). Separated proteins were transferred onto a nitrocellulose membrane using the iBlot® Dry blotting system (Invitrogen). After blocking in 5% milk for 1 hour, final sera were added at a 1:2000 dilution and incubated at +4° C. overnight. The membranes were then washed three times with PBS/0.1T followed by a one hour incubation in polyclonal rabbit anti mouse IgG conjugated to HRP (Dako) diluted 1:10,000. The immunoblots were visualized with Amersham ECL Plus™ Western blotting detection reagents (GE Healthcare) and Kodak BioMax films (Kodak).

Infection Readout

The final infection readout was based on two separate methods: detecting the presence of Borrelia-specific antibodies (western blot and VlsE ELISA) and presence of Borrelia DNA (qPCR targeting recA). In experiments where B. burgdorferi strain ZS7 was used for challenge (experiments 1 and 2), western blot together with qPCR were applied. In all other experiments, VlsE ELISA and qPCR were used. There was a high consistency between the two methods (>95%); therefore a mouse was regarded as infected when at least one of the two methods was positive. Statistical significance was determined by Fisher's exact test (two-tailed).

Results

The improved heterodimer combination vaccine was tested for protective capacity against Borrelia challenge. The results of these experiments are summarized in Table 2 Immunized mice were challenged with B. burgdorferi s.s. (OspA serotype 1, strain ZS7, needle challenge, Experiments 1 and 2 or strains Pra1 or Pra 4, tick challenge, Experiments 3 or 4 and 5, respectively), B. afzelii (OspA serotype 2, strain IS1, tick challenge, Experiments 6-9), B. garinii (OspA serotype 5, strain PHei, needle challenge, Experiments 10-13) or B. garinii (OspA serotype 6, strain Ma, needle challenge, Experiments 14-17). In some experiments, other OspA-based antigens, such as the chimera combination vaccine or a lipidated full-length OspA protein, were included. A group of mice immunized with PBS or formulation buffer combined with Al(OH)₃ served as a placebo (adjuvant alone) control group in each experiment.

The protection data from the 17 experiments are summarized in Table 2. In all experiments, a high level of infection was seen in all placebo groups. Additionally, a low infection rate was observed in the groups receiving the corresponding full length OspA protein, with the exception of the full-length OspA serotype 6, wherein only partial protection was observed (experiments 14 to 17). These results validate the experimental set-up and readout methods.

The improved heterodimer combination vaccine conferred significant protection (p-values<0.05), at a 3 μg dose, when mice were challenged with in vitro grown B. burgdorferi s.s. or B. garinii (OspA serotype 5 or 6), or ticks harboring B. burgdorferi or B. afzelii. Furthermore, when different immunization doses were assessed for vaccine efficacy, highly significant protection (p-values<0.01) could be shown when 0.03 μg of the improved heterodimer combination vaccine was administered and the mice were challenged with B. afzelii or B. garinii (OspA serotype 5 or 6) (Experiment 8, 9, 12, 13 and 16). In summary, the improved heterodimer combination vaccine induced protective immunity against three Borrelia species (B. burgdorferi, B. afzelii and B. garinii) including four clinically relevant OspA serotypes (1, 2, 5 and 6), as shown in mouse models using either in vitro grown spirochetes or infected ticks for challenge.

Protection against serotypes 5 and 6 comparable to that conferred by the improved heterodimer combination vaccine was also observed in mice immunized with the chimera combination vaccine (data not shown).

TABLE 2 Protective capacity of the improved mutant OspA heterodimer combination vaccine of the invention against OspA serotype 1, serotype 2, serotype 5 and serotype 6 Borrelia challenge. Groups of mice were immunized three times with the indicated doses of immunogen or Al(OH)₃ adjuvant alone at two-week intervals. Immunogens used were a 1:1:1 combination of the mutant OspA heterodimers Lip-S1D1-S2D1, Lip-S4D1-S3hybD1 and Lip-S5D1-S6D1 (“Improved heterodimer combination vaccine”), a 1:1:1 combination of Lip-Chimeric OspA ST1/ST2-His, Lip- Chimeric OspA ST5/ST3-His and Lip-Chimeric OspA ST6/ST4-His (“Chimera combination vaccine”) and Lip-OspA1-His (lipidated full-length OspA protein from B. burgdorferi strain B31) or Lip-OspA2-His (lipidated full-length OspA protein from B. afzelii strain K78), Lip-OspA5-His (lipidated full-length OspA protein from B. garinii strain PHei) or Lip-OspA6-His (lipidated full- length OspA protein from B. garinii strain DK29). Immunized mice were challenged s.c. two weeks after the last immunization with the indicated borrelia species using a syringe (B. burgdorferi strain ZS7, B. garinii strain PHei or B. garinii strain Ma) or using ticks (B. burgdorferi strain Pra1 or Pra4 or B. afzelii strain IS1). A Protection against needle challenge with serotype 1 OspA borrelia by chimera combination vaccine and improved heterodimer combination vaccine (one dose: 3 μg) Experiment 1: Experiment 2: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA1-His (SEQ ID NO: 34) 3 × 1.0 μg B. burgdorferi  0/10 (<0.0001)  1/10 (<0.0001) (OspA serotype 1) strain ZS7 Chimera combination vaccine: Lip-Chimeric OspA ST1/ST2-His 3 × 1.0 μg B. burgdorferi  0/10 (<0.0001)  0/10 (<0.0001) (Seq ID No: 40) (OspA serotype 1) Lip-Chimeric OspA ST5/ST3-His 3 × 1.0 μg strain ZS7 (Seq ID No: 41) Lip-Chimeric OspA ST6/ST4-His 3 × 1.0 μg (Seq ID No: 42) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg B. burgdorferi  0/10 (<0.0001)  0/10 (<0.0001) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg (OspA serotype 1) Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg strain ZS7 Al(OH)_(.) adjuvant alone — B. burgdorferi  9/9 10/10 (OspA serotype 1) strain ZS7 B Protection against tick challenge with serotype 1 OspA borrelia by chimera combination vaccine and improved heterodimer combination vaccine (one dose: 3 μg) Experiment 3: Experiment 4: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA1-His (SEQ ID NO: 34) 3 × 1.0 μg Tick challenge with  0/4 (0.0475)  1/9 (0.0216) B. burgdorferi (OspA serotype 1) strain Pra4 (Exp. 3) or Pra1 (Exp. 4) Chimera combination vaccine: Lip-Chimeric OspA ST1/ST2-His 3 × 1.0 μg Tick challenge with  0/8 (0.0060)  0/7 (0.0093) (Seq ID No: 40) B. burgdorferi Lip-Chimeric OspA ST5/ST3-His 3 × 1.0 μg (OspA serotype 1) (Seq ID No: 41) strain Pra4 (Exp. 3) Lip-Chimeric OspA ST6/ST4-His 3 × 1.0 μg or Pra1 (Exp. 4) (Seq ID No: 42) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg Tick challenge with  0/5 (0.0260)  0/7 (0.0093) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg B. burgdorferi Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg (OspA serotype 1) strain Pra4 (Exp. 3) or Pra1 (Exp. 4) Al(OH)₃ adjuvant alone — Tick challenge with  5/6  5/6 B. burgdorferi (OspA serotype 1) strain Pra4 (Exp. 3) or Pra1 (Exp. 4) C Protection against tick challenge with serotype 1 OspA borrelia by improved heterodimer combination vaccine (decreasing doses: 3 μg and 0.3 μg) Experiment 5: Infected/Total Immunogen Dose Challenge (p-value) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg Tick challenge with 1/7 (0.0174) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg B. burgdorferi Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg (OspA serotype 1) strain Pra16 Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.1 μg Tick challenge with 1/9 (0.0059) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.1 μg B. burgdorferi Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.1 μg (OspA serotype 1) strain Pral Al(OH)₃ adjuvant alone — Tick challenge with 7/8 B. burgdorferi (OspA serotype 1) strain Pral D Protection against tick challenge with serotype 2 OspA borrelia by chimera combination vaccine and improved heterodimer combination vaccine (one dose: 3 μg) Experiment 6: Experiment 7: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA2-His (SEQ ID NO: 35) 3 × 1.0 μg Tick challenge with  0/8 (0.0016)  0/9 (0.0002) B. afzelii (OspA serotype 2) strain IS1 Chimera combination vaccine: Lip-Chimeric OspA ST1/ST2-His 3 × 1.0 μg Tick challenge with  0/7 (0.0025)  0/9 (0.0002) (Seq ID No: 40) B. afzelii (OspA Lip-Chimeric OspA ST5/ST3-His 3 × 1.0 μg serotype 2) strain (Seq ID No: 41) IS1 Lip-Chimeric OspA ST6/ST4-His 3 × 1.0 μg (Seq ID No: 42) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg Tick challenge with  0/9 (0.0010)  0/7 (0.0003) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg B. afzelii (OspA Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg serotype 2) strain IS1 Al(OH)₃ adjuvant alone — Tick challenge with  5/5  8/8 B. afzelii (OspA serotype 2) strain IS1 E Protection against tick challenge with serotype 2 OspA borrelia by improved heterodimer combination vaccine (decreasing doses: 0.03 μg and 0.003 μg) Experiment 8: Experiment 9: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.01 μg Tick challenge with  0/9 (0.0004)  2/7 (0.0096) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.01 μg B. afzelii (OspA Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.01 μg serotype 2) strain IS1 Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.001 μg Tick challenge with  7/10 (n.s.)  1/8 (0.0008) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.001 μg B. afzelii (OspA Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.001 μg serotype 2) strain IS1 Al(OH)₃ adjuvant alone — Tick challenge with  6/6  9/9 B. afzelii (OspA serotype 2) strain IS1 F Protection against needle challenge with serotype 5 OspA borrelia by improved heterodimer combination vaccine (one dose: 3 μg) Experiment 10: Experiment 11: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA5-His (SEQ ID NO: 38) 3 × 1.0 μg B. garinii (OspA  0/10 (<0.0001)  0/10 (<0.0001) serotype 5) strain PHei) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg B. garinii (OspA  0/10 (<0.0001)  1/10 (<0.0001) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg serotype 5) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg PHei) Al(OH)₃ adjuvant alone — B. garinii (OspA 10/10 10/10 serotype 5) strain PHei) G Protection against needle challenge with serotype 5 OspA borrelia by improved heterodimer combination vaccine (decreasing doses: 3 μg, 0.3 μg and 0.03 μg) Experiment 12: Experiment 13: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA5-His (SEQ ID NO: 38) 3 × 1.0 μg B. garinii (OspA  0/10 (0.0007)  0/10 (<0.001) serotype 5) strain PHei) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg B. garinii (OspA  0/10 (<0.0007)  1/10 (0.0002) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg serotype 5) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg PHei) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.1 μg B. garinii (OspA  0/10 (<0.0007)  0/10 (<0.0001) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.1 μg serotype 5) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.1 μg PHei) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.01 μg B. garinii (OspA  2/10 (<0.0219)  3/10 (0.0047) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.01 μg serotype 5) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.01 μg PHei) Al(OH)₃ adjuvant alone — B. garinii (OspA  8/10  9/9 serotype 5) strain PHei) H Protection against needle challenge with serotype 6 OspA borrelia by improved heterodimer combination vaccine (one dose: 3 μg) Experiment 14: Experiment 15: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA6-His (SEQ ID NO: 39) 3 × 1.0 μg B. garinii (OspA  4/10 (0.0108)  6/10 (n.s.) serotype 6) strain Ma) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg B. garinii (OspA  0/10 (<0.0001)  0/10 (0.0007) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg serotype 6) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg Ma) Al(OH)₃ adjuvant alone — B. garinii (OspA 10/10  8/10 serotype 6) strain Ma) I Protection against needle challenge with serotype 6 OspA borrelia by improved heterodimer combination vaccine (decreasing doses: 3 μg, 0.3 μg and 0.03 μg) Experiment 16: Experiment 17: Infected/Total Infected/Total Immunogen Dose Challenge (p-value) (p-value) Lip-OspA6-His (SEQ ID NO: 39) 3 x 1.0 μg B. garinii (OspA  4/10 (0.0108)  8/10 (n.s.) serotype 6) strain Ma) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 1.0 μg B. garinii (OspA  0/10 (<0.0001)  0/10 (0.0001) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 1.0 μg serotype 6) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 1.0 μg Ma) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.1 μg B. garinii (OspA  2/10 (0.0007)  2/10 (0.0054) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.1 μg serotype 6) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.1 μg Ma) Improved heterodimer combination vaccine: Lip-S1D1-S2D1 (Seq ID No: 29) 3 × 0.01 μg B. garinii (OspA  3/10 (0.0031)  4/10 (n.s.) Lip-S4D1-S3hybD1 (Seq ID No: 27) 3 × 0.01 μg serotype 6) strain Lip-S5D1-S6D1 (Seq ID No: 33) 3 × 0.01 μg Ma) Al(OH)₃ adjuvant alone — B. garinii (OspA 10/10  9/10 serotype 6) strain Ma) P-value; Fisher's exact test, two-tailed, as compared to the adjuvant alone group, are indicated in parenthesis. not significant (n.s.).

SEQUENCES SEQ ID NO: 1 S3hybD1: hybrid OspA C-terminal fragment; amino acids of positions 125-176 from Borrelia valaisiana, strain VS116, and amino acids 177-274 from Borrelia garinii, strain PBr, with disulfide bond type 1 and T in position 233 FNEKGEVSEKILTRSNGTTLEYSQMTDAENATKAVETLKNGIKLPGNLVGGKTKLTVT C GTV TLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQK T KQLVFTKENTITVQNY NRAGNALEGSPAEIKDLAEL C AALK SEQ ID NO: 2 B. valaisiana (strain VS116), OspA aa 125-176 FNEKGEVSEKILTRSNGTTLEYSQMTDAENATKAVETLKNGIKLPGNLVGGK SEQ ID NO: 3 B. garinii (strain PBr, serotype 3), OspA aa 177-274, with T in position 233, from full-length OspA (SEQ ID NO: 8) TKLTVTCGTVTLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQK T KQLVFT KENTITVQNYNRAGNALEGSPAEIKDLAELCAALK SEQ ID NO: 4 B. valaisiana (strain VS116), OspA MKKYLLGIGLILALIACKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLVATVDKV ELKGTSDKNNGSGTLEGVKDDKSKVKLTISDDLGETKLETFKEDGTLVSRKVNFKDKSFTEE KFNEKGEVSEKILTRSNGTTLEYSQMTDAENATKAVETLKNGIKLPGNLVGGKTTLKITEGT VTLSKHIAKSGEVTVEINDTSSTPNTKKTGKWDARNSTLTIIVDSKNKTKLVFTKQDTITVQS YNPAGNKLEGTAVEIKTLQELKNALK SEQ ID NO: 5 B. burgdorferi s.s. (strain B31, OspA serotype 1) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLE LKGTSDKNNGSGVLEGVKADKSKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEE KFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTL SKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDS NGTKLEGSAVEITKLDEIKNALK SEQ ID NO: 6 B. afzelii (strain K78; OspA serotype 2) MKKYLLGIGLILALIACKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLKATVDKIE LKGTSDKDNGSGVLEGTKDDKSKAKLTIADDLSKTTFELFKEDGKTLVSRKVSSKDKTSTDE MFNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFTLEGKVANDKVTLEVKEGT VTLSKEIAKSGEVTVALNDTNTTQATKKTGAWDSKTSTLTISVNSKKTTQLVFTKQDTITVQ KYDSAGTNLEGTAVEIKTLDELKNALK SEQ ID NO: 7 B. garinii (strain PBr, OspA serotype 3) with P in position 233 (embl accession X80256.1) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGGMKVLVSKEKDKDGKYSLMATVEKL ELKGTSDKSNGSGVLEGEKADKSKAKLTISQDLNQTTFEIFKEDGKTLVSRKVNSKDKSSTEE KFNDKGKLSEKVVTRANGTRLEYTEIKNDGSGKAKEVLKGFALEGTLTDGGETKLTVTEGT VTLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQK P KQLVFTKENTITVQN YNRAGNALEGSPAEIKDLAELKAALK SEQ ID NO: 8 B. garinii (strain PBr, OspA serotype 3) with T in position 233 (embl accession ACL34827.1) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGGMKVLVSKEKDKDGKYSLMATVEKL ELKGTSDKSNGSGVLEGEKADKSKAKLTISQDLNQTTFEIFKEDGKTLVSRKVNSKDKSSTEE KFNDKGKLSEKVVTRANGTRLEYTEIKNDGSGKAKEVLKGFALEGTLTDGGETKLTVTEGT VTLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQK T KQLVFTKENTITVQN YNRAGNALEGSPAEIKDLAELKAALK SEQ ID NO: 9 B. bavariensis (strain PBi, OspA serotype 4) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGEMKVLVSKEKDKDGKYSLMATVDKL ELKGTSDKSNGSGTLEGEKSDKSKAKLTISEDLSKTTFEIFKEDGKTLVSKKVNSKDKSSIEEK FNAKGELSEKTILRANGTRLEYTEIKSDGTGKAKEVLKDFALEGTLAADKTTLKVTEGTVVL SKHIPNSGEITVELNDSNSTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQKYDSA GTNLEGNAVEIKTLDELKNALK SEQ ID NO: 10 B. garinii (strain PHei, OspA serotype 5) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGGMKVLVSKEKDKDGKYSLMATVEKL ELKGTSDKNNGSGTLEGEKTDKSKVKLTIAEDLSKTTFEIFKEDGKTLVSKKVTLKDKSSTEE KFNEKGEISEKTIVRANGTRLEYTDIKSDKTGKAKEVLKDFTLEGTLAADGKTTLKVTEGTVT LSKNISKSGEITVALDDTDSSGNKKSGTWDSGTSTLTISKNRTKTKQLVFTKEDTITVQNYDS AGTNLEGKAVEITTLKELKNALK SEQ ID NO: 11 B. garinii (strain DK29, OspA serotype 6) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGGMTVLVSKEKDKDGKYSLEATVDKLE LKGTSDKNNGSGTLEGEKTDKSKVKSTIADDLSQTKFEIFKEDGKTLVSKKVTLKDKSSTEEK FNGKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFTLEGTLAADGKTTLKVTEGTVV LSKNILKSGEITAALDDSDTTRATKKTGKWDSKTSTLTISVNSQKTKNLVFTKEDTITVQRYD SAGTNLEGKAVEITTLKELKNALK SEQ ID NO: 12 B. garinii (strain T25, OspA serotype 7) MKKYLLGIGLILALIACKQNVSSLDEKNSVSVDLPGEMKVLVSKEKDKDGKYSLEATVDKLE LKGTSDKNNGSGVLEGVKAAKSKAKLTIADDLSQTKFEIFKEDGKTLVSKKVTLKDKSSTEE KFNDKGKLSEKVVTRANGTRLEYTEIQNDGSGKAKEVLKSLTLEGTLTADGETKLTVEAGT VTLSKNISESGEITVELKDTETTPADKKSGTWDSKTSTLTISKNSQKTKQLVFTKENTITVQKY NTAGTKLEGSPAEIKDLEALKAALK SEQ ID NO: 13 Borrelia OspA lipidation signal MKKYLLGIGLILALIA SEQ ID NO: 14 Borrelia OspB lipidation signal MRLLIGFALALALIG SEQ ID NO: 15 E. coli lpp lipidation signal MKATKLVLGAVILGSTLLAG SEQ ID NO: 16 LN1 peptide linker constructed from two separate loop regions of the N-terminal half of OspA from B. burgdorferi s.s. strain B31 (aa 65-74 and aa 42-53, amino acid exchange at position 53: D53S) GTSDKNNGSGSKEKNKDGKYS SEQ ID NO: 17 hLFA-1-like sequence from B. burgdorferi s.s. strain B31 (OspA serotype 1) GYVLEGTLTAE SEQ ID NO: 18 Non-hLFA-1-like sequence from B. afzelii strain K78 (OspA serotype 2) NFTLEGKVAND SEQ ID NO: 19 B. burgdorferi s.s. (strain B31, serotype 1), OspA aa 126-273 with replaced hLFA-like sequence from serotype 1 OspA FNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKNFTLEGKVANDKTTLVVKEGTVTLS KNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSN GTKLEGSAVEITKLDEIKNALK SEQ ID NO: 20 B. afzelii (strain K78, serotype 2), OspA aa 126-273 FNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFTLEGKVANDKVTLEVKEGTV TLSKELAKSGEVTVALNDTNTTQATKKTGAWDSKTSTLTISVNSKKTTQLVFTKQDTITVQK YDSAGTNLEGTAVEIKTLDELKNALK SEQ ID NO: 21 B. garinii (strain PBr, serotype 3), OspA aa 126-274 FNDKGKLSEKVVTRANGTRLEYTEIKNDGSGKAKEVLKGFALEGTLTDGGETKLTVTEGTV TLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQKPKQLVFTKENTITVQNYN RAGNALEGSPAEIKDLAELKAALK SEQ ID NO: 22 B. bavariensis (strain PBi, serotype 4), OspA aa 126-273 FNAKGELSEKTILRANGTRLEYTEIKSDGTGKAKEVLKDFALEGTLAADKTTLKVTEGTVVL SKHIPNSGEITVELNDSNSTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQKYDSA GTNLEGNAVEIKTLDELKNALK SEQ ID NO: 23 B. garinii (strain PHei, serotype 5), OspA aa 126-273 FNEKGEISEKTIVRANGTRLEYTDIKSDKTGKAKEVLKDFTLEGTLAADGKTTLKVTEGTVTL SKNISKSGEITVALDDTDSSGNKKSGTWDSGTSTLTISKNRTKTKQLVFTKEDTITVQNYDSA GTNLEGKAVEITTLKELKNALK SEQ ID NO: 24 B. garinii (strain DK29, serotype 6), OspA aa 126-274 FNGKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFTLEGTLAADGKTTLKVTEGTVV LSKNILKSGEITAALDDSDTTRATKKTGKWDSKTSTLTISVNSQKTKNLVFTKEDTITVQRYD SAGTNLEGKAVEITTLKELKNALK SEQ ID NO: 25 B. garinii (strain T25, serotype 7) OspA aa 126-274 FNDKGKLSEKVVTRANGTRLEYTEIQNDGSGKAKEVLKSLTLEGTLTADGETKLTVEAGTVT LSKNISESGEITVELKDTETTPADKKSGTWDSKTSTLTISKNSQKTKQLVFTKENTITVQKYNT AGTKLEGSPAEIKDLEALKAALK SEQ ID NO: 26 Lip-S4D1-S3hybD1-nt Coding sequence for intermediate and final heterodimer fusion proteins of OspA serotype 4 and OspA serotype 3 with disulfide bond type 1, E. coli lpp lipidation signal, LN1 linker sequence, serotype 3 OspA fragment comprising amino acids 125-176 of B. valaisiana, strain VS116 (SEQ ID NO: 2) and amino acids 177-274 of B. garinii, strain PBr, serotype 3 (SEQ ID NO: 3) ATGAAAGCTACTAAACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGT TGCTCAAGCTTCAATGCTAAGGGCGAACTGAGCGAAAAAACGATCCTGCGTGCGAATGG CACCCGTCTGGAATACACCGAAATCAAATCCGATGGTACGGGCAAAGCAAAGGAAGTCC TGAAAGATTTTGCTCTGGAAGGTACCCTGGCGGCCGACAAAACCACGCTGAAGGTGACG TGCGGCACCGTGGTTCTGAGCAAACATATTCCGAACTCTGGTGAAATCACCGTTGAACTG AACGATAGCAATTCTACGCAGGCAACCAAAAAGACGGGCAAATGGGACAGTAATACCTC CACGCTGACCATTTCAGTCAACTCGAAAAAGACCAAAAATATTGTGTTCACGAAGGAAG ATACGATCACCGTTCAAAAATATGACTCCGCGGGCACCAACCTGGAAGGCAATGCCGTC GAAATCAAAACCCTGGATGAACTGTGTAACGCCCTGAAGGGTACTAGTGACAAAAACAA TGGCTCTGGTAGCAAAGAGAAAAACAAAGATGGCAAGTACTCATTCAACGAAAAAGGC GAAGTGAGCGAAAAAATTCTGACCCGTAGCAATGGCACCACCCTGGAATATAGCCAGAT GACCGATGCAGAAAATGCAACCAAAGCAGTTGAAACCCTGAAAAACGGTATTAAACTGC CTGGTAATCTGGTTGGTGGTAAAACCAAACTGACCGTTACCTGTGGCACCGTTACCCTGA GCAAAAACATTAGCAAAAGCGGTGAAATTACCGTGGCACTGAATGATACCGAAACCACA CCGGCAGACAAAAAAACCGGTGAATGGAAAAGCGATACCAGCACCCTGACCATTAGTAA AAATAGCCAGAAAACAAAACAGCTGGTGTTTACCAAAGAAAACACCATTACCGTGCAGA ATTATAACCGTGCAGGTAATGCACTGGAAGGTAGTCCGGCAGAAATTAAAGATCTGGCA GAACTGTGTGCAGCCCTGAAATAA SEQ ID NO: 27 Lip-S4D1-S3hybD1-aa: Heterodimer fusion protein of OspA serotype 4 and OspA serotype 3, comprising amino acids 125-176 of B. valaisiana, strain VS116 (SEQ ID NO: 2) and amino acids 177-274 of B. garinii, strain PBr, serotype 3 (SEQ ID NO: 3), with disulfide bond type 1, N-terminalCSS for addition of lipids, LN1 linker sequence, N-terminal lipidation LipCSSFNAKGELSEKTILRANGTRLEYTEIKSDGTGKAKEVLKDFALEGTLAADKTTLKVTC GTVVLSKHIPNSGEITVELNDSNSTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQ KYDSAGTNLEGNAVEIKTLDELCNALKGTSDKNNGSGSKEKNKDGKYSFNEKGEVSEKILTR SNGTTLEYSQMTDAENATKAVETLKNGIKLPGNLVGGKTKLTVTCGTVTLSKNISKSGEITV ALNDTETTPADKKTGEWKSDTSTLTISKNSQKTKQLVFTKENTITVQNYNRAGNALEGSPAEI KDLAELCAALK SEQ ID NO: 28 Lip-S1D1-S2D1-nt: Coding sequence for intermediate and final heterodimer fusion proteins of OspA serotype 1 and OspA serotype 2 with disulfide bond type 1, E. coli lpp lipidation signal, LN1 linker sequence, aa 164-174 of OspA serotype 1 replaced by non-hLFA-1-like sequence NFTLEGKVAND ATGAAAGCTACTAAACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGT TGCTCAAGCTTCAACGAAAAGGGCGAAGTCAGCGAAAAAATCATTACCCGCGCAGACGG CACCCGCCTGGAATACACCGGCATCAAATCGGACGGCAGCGGCAAAGCGAAAGAAGTTC TGAAAAACTTTACCCTGGAAGGCAAAGTCGCAAATGATAAAACCACCCTGGTGGTGAAA TGCGGCACCGTTACGCTGAGCAAAAACATTAGTAAATCCGGTGAAGTCTCTGTGGAACT GAATGATACCGACAGCTCTGCGGCCACCAAGAAAACCGCAGCTTGGAACTCAGGCACCT CGACGCTGACCATTACGGTTAATAGCAAGAAAACCAAAGATCTGGTCTTCACGAAAGAA AACACCATCACGGTGCAGCAATATGACAGCAATGGTACCAAACTGGAAGGCTCCGCTGT GGAAATCACGAAACTGGATGAAATCTGTAATGCTCTGAAAGGTACTAGTGACAAAAACA ATGGCTCTGGTAGCAAAGAGAAAAACAAAGATGGCAAGTACTCATTCAACGAAAAAGG CGAACTGTCGGCGAAAACGATGACGCGTGAAAACGGCACCAAACTGGAATATACGGAA ATGAAAAGCGATGGCACCGGTAAAGCGAAAGAAGTTCTGAAAAACTTTACCCTGGAAGG CAAAGTCGCCAATGACAAAGTCACCCTGGAAGTGAAATGCGGCACCGTTACGCTGTCAA AAGAAATTGCAAAATCGGGTGAAGTGACCGTTGCTCTGAACGATACGAATACCACGCAA GCGACCAAGAAAACCGGCGCCTGGGACAGCAAAACCTCTACGCTGACCATTAGTGTTAA TAGCAAGAAAACCACGCAGCTGGTCTTCACCAAACAAGATACGATCACCGTGCAGAAAT ACGACAGTGCGGGTACCAACCTGGAAGGCACGGCTGTTGAAATCAAAACCCTGGACGAA CTGTGTAACGCCCTGAAA SEQ ID NO: 29 Lip-S1D1-S2D1-aa: Heterodimer fusion protein of OspA serotype 1 and OspA serotype 2 with disulfide bond type 1, N-terminal CSS for addition of lipids, LN1 linker sequence, aa 164-174 of OspA serotype 1 replaced by non-hLFA-1-like sequence NFTLEGKVAND, N-terminal lipidation LipCSSFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKNFTLEGKVANDKTTLVVKC GTVTLSKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITV QQYDSNGTKLEGSAVEITKLDEICNALKGTSDKNNGSGSKEKNKDGKYSFNEKGELSAKTM TRENGTKLEYTEMKSDGTGKAKEVLKNFTLEGKVANDKVTLEVKCGTVTLSKEIAKSGEVT VALNDTNTTQATKKTGAWDSKTSTLTISVNSKKTTQLVFTKQDTITVQKYDSAGTNLEGTAV EIKTLDELCNALK SEQ ID NO: 30 Lip-S4D1-S3D1-nt: Coding sequence for intermediate and final heterodimer fusion proteins of OspA serotypes 4 and 3 both with disulfide bond type 1, E. coli lpp lipidation signal, N-terminal CSS for addition of lipids, LN1 linker sequence ATGAAAGCTACTAAACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGT TGCTCAAGCTTCAATGCTAAGGGCGAACTGAGCGAAAAAACGATCCTGCGTGCGAATGG CACCCGTCTGGAATACACCGAAATCAAATCCGATGGTACGGGCAAAGCAAAGGAAGTCC TGAAAGATTTTGCTCTGGAAGGTACCCTGGCGGCCGACAAAACCACGCTGAAGGTGACG TGCGGCACCGTGGTTCTGAGCAAACATATTCCGAACTCTGGTGAAATCACCGTTGAACTG AACGATAGCAATTCTACGCAGGCAACCAAAAAGACGGGCAAATGGGACAGTAATACCTC CACGCTGACCATTTCAGTCAACTCGAAAAAGACCAAAAATATTGTGTTCACGAAGGAAG ATACGATCACCGTTCAAAAATATGACTCCGCGGGCACCAACCTGGAAGGCAATGCCGTC GAAATCAAAACCCTGGATGAACTGTGTAACGCCCTGAAGGGTACTAGTGACAAAAACAA TGGCTCTGGTAGCAAAGAGAAAAACAAAGATGGCAAGTACTCATTTAACGATAAGGGCA AACTGTCGGAAAAAGTGGTCACCCGCGCAAATGGCACCCGCCTGGAATACACGGAAATC AAAAACGATGGTAGCGGCAAAGCGAAGGAAGTTCTGAAAGGCTTTGCCCTGGAAGGTAC CCTGACGGATGGCGGTGAAACCAAACTGACCGTGACGTGCGGCACCGTTACGCTGTCTA AAAACATTAGCAAGTCTGGTGAAATCACGGTCGCACTGAATGATACCGAAACCACGCCG GCTGACAAAAAGACCGGCGAATGGAAAAGTGACACCTCCACGCTGACCATTTCAAAGAA CTCGCAGAAACCGAAGCAACTGGTCTTCACCAAAGAAAACACGATCACCGTGCAGAACT ATAATCGTGCCGGTAATGCTCTGGAAGGCTCACCGGCTGAAATCAAGGACCTGGCTGAA CTGTGTGCGGCACTGAAA SEQ ID NO: 31 Lip-S4D1-S3D1-aa: Heterodimer fusion protein of OspA serotypes 4 and 3 both with disulfide bond type 1, N-terminal CSS for addition of lipids, LN1 linker sequence, N-terminal lipidation LipCSSFNAKGELSEKTILRANGTRLEYTEIKSDGTGKAKEVLKDFALEGTLAADKTTLKVTC GTVVLSKHIPNSGEITVELNDSNSTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQ KYDSAGTNLEGNAVEIKTLDELCNALKGTSDKNNGSGSKEKNKDGKYSFNDKGKLSEKVVT RANGTRLEYTEIKNDGSGKAKEVLKGFALEGTLTDGGETKLTVTCGTVTLSKNISKSGEITVA LNDTETTPADKKTGEWKSDTSTLTISKNSQKPKQLVFTKENTITVQNYNRAGNALEGSPAEIK DLAELCAALK SEQ ID NO: 32 Lip-S5D1-S6D1-nt: Coding sequence for intermediate and final heterodimer fusion proteins of OspA serotypes 6 both with disulfide bond type 1, E. coli lpp lipidation signal, N-terminal CSS for addition of lipids, LN1 linker sequence ATGAAAGCTACTAAACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGT TGCTCAAGCTTCAACGAAAAGGGCGAAATCTCAGAAAAAACCATCGTCCGCGCTAACGG CACCCGCCTGGAATACACCGACATCAAATCAGACAAGACCGGTAAAGCGAAGGAAGTTC TGAAAGATTTTACGCTGGAAGGTACCCTGGCAGCAGACGGTAAAACCACGCTGAAGGTG ACCTGCGGTACCGTTACGCTGTCCAAAAACATTAGTAAGTCCGGCGAAATCACGGTCGCC CTGGATGACACCGATAGCTCTGGCAACAAAAAGAGCGGTACCTGGGATTCAGGCACCTC GACGCTGACCATTTCTAAAAATCGTACGAAAACCAAGCAGCTGGTCTTCACGAAAGAAG ATACGATCACCGTGCAAAACTATGACAGCGCAGGTACCAATCTGGAAGGCAAAGCTGTG GAAATTACCACGCTGAAAGAACTGTGTAATGCTCTGAAAGGTACTAGTGACAAAAACAA TGGCTCTGGTAGCAAAGAGAAAAACAAAGATGGCAAGTACTCATTCAACGGCAAAGGTG AAACGAGCGAAAAGACCATCGTGCGTGCGAACGGTACCCGCCTGGAATATACGGACATT AAATCGGACGGCAGCGGCAAAGCAAAGGAAGTCCTGAAAGATTTTACGCTGGAAGGTAC CCTGGCAGCAGACGGTAAAACCACGCTGAAGGTGACGTGCGGCACCGTGGTTCTGTCAA AAAACATTCTGAAGTCGGGTGAAATCACCGCAGCTCTGGATGACAGCGATACCACGCGT GCTACGAAAAAGACCGGTAAATGGGATAGCAAGACCTCTACGCTGACCATTAGTGTCAA CTCCCAGAAAACGAAGAATCTGGTGTTCACCAAAGAAGATACGATCACCGTTCAACGCT ATGACAGTGCGGGCACCAACCTGGAAGGCAAAGCCGTTGAAATTACCACGCTGAAAGAA CTGTGTAATGCTCTGAAA SEQ ID NO: 33 Lip-S5D1-S6D1-aa: Heterodimer fusion protein of OspA serotypes 6 both with disulfide bond type 1, N-terminal CSS for addition of lipids, LN1 linker sequence, N-terminal lipidation LipCSSFNEKGEISEKTIVRANGTRLEYTDIKSDKTGKAKEVLKDFTLEGTLAADGKTTLKVTC GTVTLSKNISKSGEITVALDDTDSSGNKKSGTWDSGTSTLTISKNRTKTKQLVFTKEDTITVQ NYDSAGTNLEGKAVEITTLKELCNALKGTSDKNNGSGSKEKNKDGKYSFNGKGETSEKTIVR ANGTRLEYTDIKSDGSGKAKEVLKDFTLEGTLAADGKTTLKVTCGTVVLSKNILKSGEITAA LDDSDTTRATKKTGKWDSKTSTLTISVNSQKTKNLVFTKEDTITVQRYDSAGTNLEGKAVEI TTLKELCNALK SEQ ID NO: 34 B. burgdorferi (strain B31, OspA serotype 1) aa 18-273, lpp lipidation signal sequence removed (MKATKLVLGAVILGSTLLAG, SEQ ID NO: 15), C-terminal His tag (LEHHHHHH), N-terminal CSSF for addition of lipids CSSFKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSG VLEGVKADKSKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKII TRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSV ELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEI TKLDEIKNALKLEHHHHHH SEQ ID NO: 35 B. afzelii (strain K78; OspA serotype 2) aa 18-273, lpp lipidation signal sequence removed (MKATKLVLGAVILGSTLLAG, SEQ ID NO: 15), C-terminal His tag (LEHHHHHH), N-terminal CSSF for addition of lipids CSSFKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLKATVDKIELKGTSDKDNGSG VLEGTKDDKSKAKLTIADDLSKTTFELFKEDGKTLVSRKVSSKDKTSTDEMFNEKGELSAKT MTRENGTKLEYTEMKSDGTGKAKEVLKNFTLEGKVANDKVTLEVKEGTVTLSKEIAKSGEV TVALNDTNTTQATKKTGAWDSKTSTLTISVNSKKTTQLVFTKQDTITVQKYDSAGTNLEGTA VEIKTLDELKNALKLEHHHHHH SEQ ID NO: 36 B. garinii (strain PBr; OspA serotype 3) aa 18-274, lpp lipidation signal sequence removed (MKATKLVLGAVILGSTLLAG, SEQ ID NO: 15), C-terminal His tag (LEHHHHHH), N-terminal CSSF for addition of lipids CSSFKQNVSSLDEKNSVSVDLPGGMKVLVSKEKDKDGKYSLMATVEKLELKGTSDKSNGSG VLEGEKADKSKAKLTISQDLNQTTFEIFKEDGKTLVSRKVNSKDKSSTEEKFNDKGKLSEKV VTRANGTRLEYTEIKNDGSGKAKEVLKGFALEGTLTDGGETKLTVTEGTVTLSKNISKSGEIT VALNDTETTPADKKTGEWKSDTSTLTISKNSQKTKQLVFTKENTITVQNYNRAGNALEGSPA EIKDLAELKAALKLEHHHHHH SEQ ID NO: 37 B. bavariensis (strain PBi; OspA serotype 4) aa 18-273, lpp lipidation signal sequence removed (MKATKLVLGAVILGSTLLAG, SEQ ID NO: 15), C-terminal His tag (LEHHHHHH), N-terminal CSSF for addition of lipids CSSFKQNVSSLDEKNSVSVDLPGEMKVLVSKEKDKDGKYSLMATVDKLELKGTSDKSNGSG TLEGEKSDKSKAKLTISEDLSKTTFEIFKEDGKTLVSKKVNSKDKSSIEEKFNAKGELSEKTILR ANGTRLEYTEIKSDGTGKAKEVLKDFALEGTLAADKTTLKVTEGTVVLSKHIPNSGEITVELN DSNSTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQKYDSAGTNLEGNAVEIKTL DELKNALKLEHHHHHH SEQ ID NO: 38 B. garinii (strain PHei; OspA serotype 5) aa 18-273, lpp lipidation signal sequence removed (MKATKLVLGAVILGSTLLAG, SEQ ID NO: 15), C-terminal His tag (LEHHHHHH, SEQ ID NO: 64), N-terminal CSSF (SEQ ID NO: 65) for addition of lipids CSSFKQNVSSLDEKNSVSVDLPGGMKVLVSKEKDKDGKYSLMATVEKLELKGTSDKNNGSG TLEGEKTDKSKVKLTIAEDLSKTTFEIFKEDGKTLVSKKVTLKDKSSTEEKFNEKGEISEKTIV RANGTRLEYTDIKSDKTGKAKEVLKDFTLEGTLAADGKTTLKVTEGTVTLSKNISKSGEITVA LDDTDSSGNKKSGTWDSGTSTLTISKNRTKTKQLVFTKEDTITVQNYDSAGTNLEGKAVEITT LKELKNALKLEHHHHHH SEQ ID NO: 39 B. garinii (strain DK29; OspA serotype 6) aa 18-274, lpp lipidation signal sequence removed (MKATKLVLGAVILGSTLLAG, SEQ ID NO: 15), C-terminal His tag (LEHHHHHH), N-terminal CSSF for addition of lipids CSSFKQNVSSLDEKNSVSVDLPGGMTVLVSKEKDKDGKYSLEATVDKLELKGTSDKNNGSG TLEGEKTDKSKVKSTIADDLSQTKFEIFKEDGKTLVSKKVTLKDKSSTEEKFNGKGETSEKTI VRANGTRLEYTDIKSDGSGKAKEVLKDFTLEGTLAADGKTTLKVTEGTVVLSKNILKSGEIT AALDDSDTTRATKKTGKWDSKTSTLTISVNSQKTKNLVFTKEDTITVQRYDSAGTNLEGKAV EITTLKELKNALKLEHHHHHH SEQ ID NO: 40 Chimeric OspA Serotype1/Serotype2, N-terminal lipidation, His-tagged, including the OspB lipidation signal sequence: MRLLIGFALALALIG (SEQ ID NO: 14) which is cleaved during processing MRLLIGFALALALIGCAQKGAESIGSVSVDLPGEMKVLVSKEKDKNGKYDLIATVDKLELKG TSDKNNGSGVLEGVKTNKSKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFN EKGEVSEKIITMADGTRLEYTGIKSDGTGKAKYVLKNFTLEGKVANDKTTLEVKEGTVTLSM NISKSGEVSVELNDTDSSAATKKTAAWNSKTSTLTISVNSKKTTQLVFTKQDTITVQKYDSAG TNLEGTAVEIKTLDELKNALKLEHHHHHH SEQ ID NO: 41 Chimeric OspA Serotype5/Serotype3, N-terminal lipidation, His-tagged, including the OspB lipidation signal sequence: MRLLIGFALALALIG (SEQ ID NO: 14) which is cleaved during processing MRLLIGFALALALIGCAQKGAESIGSVSVDLPGGMKVLVSKEKDKNGKYSLMATVEKLELK GTSDKNNGSGTLEGEKTNKSKVKLTIAEDLSKTTFEIFKEDGKTLVSKKVTLKDKSSTEEKFN EKGEISEKTIVMANGTRLEYTDIKSDKTGKAKYVLKDFTLEGTLAADGKTTLKVTEGTVTLS MNISKSGEITVALDDTDSSGNKKSGTWDSDTSTLTISKNSQKTKQLVFTKENTITVQNYNRAG NALEGSPAEIKDLAELKAALKLEHHHHHH SEQ ID NO: 42 Chimeric OspA Serotype6/Serotype4, N-terminal lipidation, His-tagged, including the OspB lipidation signal sequence: MRLLIGFALALALIG (SEQ ID NO: 14) which is cleaved during processing MRLLIGFALALALIGCAQKGAESIGSVSVDLPGGMTVLVSKEKDKNGKYSLEATVDKLELKG TSDKNNGSGTLEGEKTNKSKVKLTIADDLSQTKFEIFKEDAKTLVSKKVTLKDKSSTEEKFNE KGETSEKTIVMANGTRLEYTDIKSDGSGKAKYVLKDFTLEGTLAADGKTTLKVTEGTVVLS MNILKSGEITVALDDSDTTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQKYDSA GTNLEGNAVEIKTLDELKNALKLEHHHHHH SEQ ID NO: 43 S1D1 FNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKNFTLEGKVANDKTTLVVKCGTVTL SKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDS NGTKLEGSAVEITKLDEICNALK SEQ ID NO: 44 S2D1 FNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFTLEGKVANDKVTLEVKCGTV TLSKEIAKSGEVTVALNDTNTTQATKKTGAWDSKTSTLTISVNSKKTTQLVFTKQDTITVQK YDSAGTNLEGTAVEIKTLDELCNALK SEQ ID NO: 45 S3D1 FNDKGKLSEKVVTRANGTRLEYTEIKNDGSGKAKEVLKGFALEGTLTDGGETKLTVTCGTV TLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQKTKQLVFTKENTITVQNY NRAGNALEGSPAEIKDLAELCAALK SEQ ID NO: 46 S4D1 FNAKGELSEKTILRANGTRLEYTEIKSDGTGKAKEVLKDFALEGTLAADKTTLKVTCGTVVL SKHIPNSGEITVELNDSNSTQATKKTGKWDSNTSTLTISVNSKKTKNIVFTKEDTITVQKYDSA GTNLEGNAVEIKTLDELCNALK SEQ ID NO: 47 S5D1 FNEKGEISEKTIVRANGTRLEYTDIKSDKTGKAKEVLKDFTLEGTLAADGKTTLKVTCGTVTL SKNISKSGEITVALDDTDSSGNKKSGTWDSGTSTLTISKNRTKTKQLVFTKEDTITVQNYDSA GTNLEGKAVEITTLKELCNALK SEQ ID NO: 48 S6D1 FNGKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFTLEGTLAADGKTTLKVTCGTVV LSKNILKSGEITAALDDSDTTRATKKTGKWDSKTSTLTISVNSQKTKNLVFTKEDTITVQRYD SAGTNLEGKAVEITTLKELCNALK SEQ ID NO: 49 S3HYBD1 (BVA) FNEKGEVSEKILTRSNGTTLEYSQMTDAENATKAVETLKNGIKLPGNLVGGKTKLTVTCGTV TLSKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQKTKQLVFTKENTITVQNY NRAGNALEGSPAEIKDLAELCAALK SEQ ID NO: 50 BVAD1 FNEKGEVSEKILTRSNGTTLEYSQMTDAENATKAVETLKNGIKLPGNLVGGKTTLKITCGTVT LSKHIAKSGEVTVEINDTSSTPNTKKTGKWDARNSTLTIIVDSKNKTKLVFTKQDTITVQSYNP AGNKLEGTAVEIKTLQELCNALK SEQ ID NO: 51 S3hybD1(Bsp): hybrid OspA C-terminal fragment; amino acids 126-175 from Borrelia spielmanii and amino acids 177-274 from Borrelia garinii, strain PBr, with disulfide bond type 1 and T in position 233 FNEKGELSEKTLVRANGTKLEYTEIKSDGTGKAKEVLKDFTLEGTLANEKTKLTVT C GTVTL SKNISKSGEITVALNDTETTPADKKTGEWKSDTSTLTISKNSQK T KQLVFTKENTITVQNYNR AGNALEGSPAEIKDLAEL C AALK SEQ ID NO: 52 MSPD1 FNEKGELSEKTLVRANGTKLEYTEIKSDGTGKAKEVLKDFTLEGTLANEKATLTVKCGTVTL SKNIDKSGEVTVALNDTDSTAATKKTGAWDSKTSTLTITVNSKKTKDLVFTKQDTITVQKYD SAGTTLEGSAVEIKTLDELCNALK SEQ ID NO: 53 Forward primer for the 16S-23S intergenic spacer GTATGTTTAGTGAGGGGGGTG SEQ ID NO: 54 Reverse primer for the 16S-23S intergenic spacer GGATCATAGCTCAGGTGGTTAG SEQ ID NO: 55 Forward nested primer for the 16S-23S intergenic spacer AGGGGGGTGAAGTCGTAACAAG SEQ ID NO: 56 Reverse nested primer for the 16S-23S intergenic spacer GTCTGATAAACCTGAGGTCGGA SEQ ID NO: 57 Forward primer for the RecA gene of Borrelia CATGCTCTTGATCCTGTTTA SEQ ID NO: 58 Reverse primer for the RecA gene of Borrelia CCCATTTCTCCATCTATCTC SEQ ID NO: 59 25-mer peptide from the Invariable Region 6 (IR6) of VlSE MKKDDQIAAAMVLRGMAKDGQFALK SEQ ID NO: 60 Mouse cathelin RLAGLLRKGGEKIGEKLKKIGQKIKNFFQKLVPQPE SEQ ID NO: 61 KLK peptide KLKLLLLLKLK SEQ ID NO: 62 N-terminal peptide for lipidation CKQN SEQ ID NO: 63 5′-(dIdC)₁₃-3′ dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC

The entire contents of all of the references (including literature references, issued patents, published patent applications, and co-pending patent applications) cited throughout this application are hereby expressly incorporated by reference. 

1.-49. (canceled)
 50. A method of producing a polypeptide, the method comprising: (a) introducing a vector encoding the polypeptide into a host cell; (b) growing the host cell under conditions allowing for expression of the polypeptide; wherein the polypeptide comprises a first disulfide bond-stabilized C-terminal fragment of an outer surface protein A (OspA), wherein the first disulfide bond-stabilized C-terminal fragment is a hybrid C-terminal OspA fragment consisting of, from the N- to C-terminal direction, a fusion of a first and a second OspA portion from two different Borrelia strains, and wherein: (i) the first OspA portion consists of amino acids 125-176 or amino acids 126-175 of OspA from a Borrelia strain that is not the corresponding fragment of B. garinii, strain PBr OspA with SEQ ID NO: 8, wherein the numbering of amino acids is according to the numbering of corresponding amino acids of the full-length OspA of B. burgdoferi s.s., strain B31 with SEQ ID NO: 5; and (ii) the second OspA portion consists of amino acids 176-274 or amino acids 177-274 of OspA from B. garinii, strain PBr (SEQ ID NO: 8), wherein the second OspA portion differs from the corresponding wild-type sequence of SEQ ID NO: 8 at least by: (1) substitution of the wild-type amino acid at position 183+/−3 of SEQ ID NO: 8 by a cysteine, and (2) substitution of the wild-type amino acid at position 270+/−3 of SEQ ID NO: 8 by a cysteine, wherein a disulfide bond is present between the introduced cysteines.
 51. The method of claim 50, wherein the hybrid C-terminal OspA fragment comprises the amino acid sequence defined by SEQ ID NO:
 1. 52. The method of claim 50, wherein the hybrid C-terminal OspA fragment comprises the amino acid sequence defined by SEQ ID NO:
 51. 53. The method of claim 50, wherein the second OspA portion comprises a substitution of a threonine residue at position 233 of wild-type OspA of B. garinii, strain PBr, as defined by SEQ ID NO: 8, with a proline residue.
 54. The method of claim 50, wherein the polypeptide further comprises a second disulfide bond-stabilized C-terminal OspA fragment; wherein said second disulfide bond-stabilized C-terminal OspA fragment consists of a C-terminal domain of an OspA from B. burgdorferi s.s., B. afzelii, B. bavariensis, or B. garinii, which differs from the corresponding wild-type OspA sequence at least by the introduction of at least one disulfide bond, and wherein said second disulfide bond-stabilized C-terminal OspA fragment is not a hybrid C-terminal OspA fragment.
 55. The method of claim 54, wherein said at least one disulfide bond is formed by the substitution of the amino acid at position 182+/−3 of the wild-type sequence by a cysteine and by the substitution of the amino acid at position 269+/−3 of the wild-type sequence by a cysteine; and wherein the numbering of said amino acids is according to the numbering of corresponding amino acids of the full length OspA of B. burgdorferi s.s., strain B31 (SEQ ID NO: 5).
 56. The method of claim 54, wherein the polypeptide comprises a linker between the hybrid C-terminal OspA fragment and the second C-terminal OspA fragment, optionally wherein the linker comprises linker comprises the amino acid sequence (SEQ ID NO: 16) GTSDKNNGSGSKEKNKDGKYS


57. The method of claim 50, wherein the polypeptide comprises an E. coli-derived 1pp lipidation signal as defined by MKATKLVLGAVILGSTLLAG (SEQ ID NO: 15); or the polypeptide comprises a lipidation site peptide led by an N-terminal cysteine residue as a site for lipidation, wherein said lipidation site peptide is defined by the amino acid sequence CSS.
 58. The method of claim 50, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
 27. 59. The method of claim 50, wherein the polypeptide consists of the amino acid sequence of SEQ ID NO:
 27. 60. The method of claim 50, wherein the vector comprises the nucleic acid sequence of SEQ ID NO: 26, optionally wherein the vector is pET28b(+).
 61. The method of claim 50, wherein the host cell is an E. coli cell, optionally an E. coli BL21 cell.
 62. The method of claim 50, further comprising (c) homogenizing the host cell to produce a host cell homogenate.
 63. The method of claim 62, further comprising (d) subjecting the host cell homogenate to one or more purification steps.
 64. The method of claim 50, wherein the one or more purification steps comprise enriching the polypeptide in a lipid phase separation and purifying the polypeptide over a gel filtration column.
 65. The method of claim 64, wherein the one or more purification steps further comprise processing the polypeptide over a buffer exchange column.
 66. A method for producing a pharmaceutical composition comprising combining a polypeptide with a pharmaceutically acceptable excipient, wherein the polypeptide comprises a first disulfide bond-stabilized C-terminal fragment of an outer surface protein A (OspA), wherein the first disulfide bond-stabilized C-terminal fragment is a hybrid C-terminal OspA fragment consisting of, from the N- to C-terminal direction, a fusion of a first and a second OspA portion from two different Borrelia strains, wherein the polypeptide induces an immune response protective against a Borrelia infection, and wherein: (i) the first OspA portion consists of amino acids 125-176 or amino acids 126-175 of OspA from a Borrelia strain that is not the corresponding fragment of B. garinii, strain PBr OspA with SEQ ID NO: 8, wherein the numbering of amino acids is according to the numbering of corresponding amino acids of the full-length OspA of B. burgdoferi s.s., strain B31 with SEQ ID NO: 5; and (ii) the second OSpA portion consists of amino acids 176-274 or amino acids 177-274 of OspA from B. garinii, strain PBr (SEQ ID NO: 8), wherein the second OspA portion differs from the corresponding wild-type sequence of SEQ ID NO: 8 at least by: (1) substitution of the wild-type amino acid at position 183+/−3 of SEQ ID NO: 8 by a cysteine, and (2) substitution of the wild-type amino acid at position 270+/−3 of SEQ ID NO: 8 by a cysteine, wherein a disulfide bond is present between the introduced cysteines.
 67. The method of claim 66, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
 27. 68. The method of claim 66, further comprising combining the polypeptide and pharmaceutically acceptable excipient with: (i) a second polypeptide comprising the amino acid sequence of SEQ ID NO: 29; and/or (ii) a third polypeptide comprising the amino acid sequence of SEQ ID NO:
 33. 69. The method of claim 66, wherein the pharmaceutically acceptable excipient comprises L-methionine and/or aluminium hydroxide.
 70. The method of claim 66, wherein the pharmaceutical composition is a vaccine. 